猪腰椎间盘的水合状态:新鲜标本与冷冻解冻标本的比较

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL Annals of Biomedical Engineering Pub Date : 2024-07-16 DOI:10.1007/s10439-024-03577-y
Concetta Morino, Jason Kait, Cameron R Bass
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引用次数: 0

摘要

椎间盘(IVD)中的含水量对生理和机械功能至关重要。在生物力学测试前冷冻死后组织是防止组织降解的常见做法,但理论上这一过程会改变 IVD 内的水合状态。猪腰部 IVD 是一种常见的腰部替代组织,其内部的水合状态以及冷冻对猪 IVD 水合状态的影响尚不清楚。19 个猪腰椎被储存在三种条件之一:用盐水浸湿的纱布包裹冷冻(- 20 °C)、不含盐水的冷冻(- 20 °C)或新鲜。对 89 个椎间盘中的四个椎间盘区域进行了含水量测量:髓核(NP)、内侧(AF-A)、中间(AF-B)和外侧(AF-C)纤维环。对储存条件、脊柱水平和重复测量的椎间盘区域进行了三因素重复测量方差分析。作为主效应的脊柱水平或储存条件没有观察到明显差异。各椎间盘区域的平均水合度存在显著差异,水的质量百分比分别为:NP(88.8 ± 1.7%)、AF-A(79.6 ± 3.8%)、AF-B(71.9 ± 3.7%)和 AF-C(62.3 ± 3.3%)。在不同的储存条件下,NP 和 AF-C 区域无明显差异。在 AF-A 和 AF-B 区域观察到两种明显的储存条件差异,但这些种群可能没有生物差异。测定了整个猪腰椎内膜的含水量,结果表明-20 °C的一个冻融循环不会改变猪腰椎内膜的整体水合作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Hydration State Throughout Porcine Lumbar Intervertebral Discs: Comparing Fresh and Frozen-Thawed Specimens.

Water content in intervertebral discs (IVDs) is essential for physiological and mechanical function. Freezing post-mortem tissue prior to biomechanical testing is a common practice to prevent tissue degradation, but this process has been theorized to alter hydration within IVDs. The hydration state throughout porcine lumbar IVDs, a common lumbar surrogate, is unknown as are the effects of freezing on porcine IVD hydration. Nineteen porcine lumbar spines were stored in one of the three conditions: frozen (- 20 °C) wrapped in saline-soaked gauze, frozen (- 20 °C) without saline, or fresh. Water content was measured in four disc regions within each of 89 discs: nucleus pulposus (NP), inner (AF-A), intermediate (AF-B), and outer (AF-C) annulus fibrosus. A three-factor, repeated measure analysis of variance was conducted for storage condition, spinal level, and repeated measure disc region. No significant differences were observed in spinal level or storage condition as a main effect. Mean hydration was significantly different in each disc region with mass percentage of water found to be 88.8 ± 1.7% in NP, 79.6 ± 3.8% in AF-A, 71.9 ± 3.7% in AF-B, and 62.3 ± 3.3% in AF-C. No significant differences were shown in NP and AF-C regions between storage conditions. Two significant differences in storage condition were observed in AF-A and AF-B regions, but there is likely no biological difference in these populations. Water content throughout porcine lumbar IVD was determined and results suggest one freeze-thaw cycle at - 20 °C does not alter the overall hydration within the porcine lumbar IVD.

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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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