通过向血液中注射新鲜分离的心脏线粒体进行光疗,可降低磷化铝诱导的死亡率,并保护心脏组织免受氧化应激和线粒体损伤。

IF 3.4 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Cardiovascular Toxicology Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI:10.1007/s12012-024-09896-9
Mohammad Shabani, Saleh Khezri, Ahmad Salimi
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引用次数: 0

摘要

磷化铝(AlP)中毒的特征是受害者心力衰竭,这与活性氧(ROS)、线粒体功能障碍、氧化应激、抗氧化防御系统的改变以及心肌细胞中 ATP 的耗竭有关。在本研究中,我们假设向血液中注入分离的线粒体或线粒体移植可能会成为 AlP 释放的磷化氢的主要靶点,并抑制 AlP 引起的大鼠死亡和心脏毒性。健康成年雄性 Wistar 大鼠被随机分为 5 组,分别为对照组、AlP 组(12.5 毫克/千克,口服)、AlP + 线粒体组(125 微克/千克)、AlP + 线粒体组(250 微克/千克)和单独线粒体组(250 微克/千克)。从大鼠心脏中分离出功能完整的线粒体,并在接触 AlP 30 分钟后通过尾静脉进行移植。在 30 天内对存活率、组织病理学改变、心脏生化指标、氧化应激和线粒体毒性参数进行了监测和分析。我们发现,向血液中注射浓度为 125 和 250 125 µg/ml 的健康线粒体可显著提高大鼠的存活率,30 天内存活率分别达到 40% 和 56.25%。此外,我们还观察到,向血液中注入线粒体可减少组织病理学损伤、心脏生化指标、氧化应激和线粒体毒性参数。据我们所知,本研究是文献中首次报道线粒体移植对 AlP 引起的死亡和心脏毒性有良好的治疗效果。本研究结果表明,向血液中注入外源性线粒体可能是治疗 AlP 中毒的一种有效治疗策略。
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Mitotherapy with Fresh Isolated Cardiac Mitochondria Via Injection Into Blood Reduces Aluminum Phosphide-Induced Mortality and Protects Cardiac Tissue Against Oxidative Stress and Mitochondrial Damages.

The hallmark of aluminum phosphide (AlP) poisoning is heart failure in victims which is associated with reactive oxygen species (ROS), mitochondrial dysfunction, oxidative stress, alteration in antioxidant defense system and depletion of ATP in cardiomyocytes. In the present study, we hypothesized that the injection of isolated mitochondria into blood or mitochondrial transplantation can likely create a primary target for phosphine released from AlP and inhibit AlP-induced mortality and cardiotoxicity in rat. Male, Wistar, healthy and adult rats were randomly divided into 5 groups as control, AlP (12.5 mg/kg, orally), AlP + mitochondria (125 µg/kg), AlP + mitochondria (250 µg/kg) and mitochondria (250 µg/kg) alone. Functional and intact mitochondria isolated from rat heart and transplantation was carried out via tail vein, 30 min after exposure to AlP. Survival rate, histopathological alterations, cardiac biochemical markers, oxidative stress and mitochondrial toxicity parameters were monitored and analyzed during 30 days. We found that injection of healthy mitochondria into blood at concentrations of 125 and 250 125 µg/ml significantly increased the survival of rats up to 40% and 56.25% respectively, during 30 days. Moreover, we observed that mitochondria injection into blood decreased histopathological damages, cardiac biochemical markers, oxidative stress and mitochondrial toxicity parameters. To our knowledge, the current study is the first report in the literature that demonstrated good therapeutic effects of mitochondrial transplantation in AlP-induced mortality and cardiotoxicity. The findings of the present study suggests that injection of exogenous mitochondria into blood could be an effective therapeutic strategy in treating AlP poisoning.

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来源期刊
Cardiovascular Toxicology
Cardiovascular Toxicology 医学-毒理学
CiteScore
6.60
自引率
3.10%
发文量
61
审稿时长
>12 weeks
期刊介绍: Cardiovascular Toxicology is the only journal dedicated to publishing contemporary issues, timely reviews, and experimental and clinical data on toxicological aspects of cardiovascular disease. CT publishes papers that will elucidate the effects, molecular mechanisms, and signaling pathways of environmental toxicants on the cardiovascular system. Also covered are the detrimental effects of new cardiovascular drugs, and cardiovascular effects of non-cardiovascular drugs, anti-cancer chemotherapy, and gene therapy. In addition, Cardiovascular Toxicology reports safety and toxicological data on new cardiovascular and non-cardiovascular drugs.
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