表达重组尖峰蛋白的多种方法：全面回顾。

IF 1.4 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Protein expression and purification Pub Date : 2024-07-14 DOI:10.1016/j.pep.2024.106556

Jk Nithya Shree, T. Premika, S. Sharlin, A. Annie Aglin

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引用次数: 0

摘要

严重急性呼吸系统综合症冠状病毒-2（SARS-CoV-2）的尖峰蛋白负责感染宿主细胞。它有两个片段，即 S1 和 S2。S1 段有一个受体结合域（RBD），可与宿主受体血管紧张素转换酶 2（ACE2）结合。S2 段通过两个七重复结构域形成六螺旋束，有助于病毒细胞膜的融合。要开发针对 COVID-19 的有效疫苗和疗法，表达和纯化 SARS-CoV-2 Spike 蛋白至关重要。目前已对完整重组尖峰蛋白或其片段的表达进行了广泛研究。本综述深入分析了用于表达尖峰蛋白的不同表达系统及其优缺点。

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Diverse approaches to express recombinant spike protein: A comprehensive review

The spike protein of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for infecting host cells. It has two segments, S1 and S2. The S1 segment has a receptor-binding domain (RBD) that attaches to the host receptor angiotensin-converting enzyme 2 (ACE2). The S2 segment helps in the fusion of the viral cell membrane by creating a six-helical bundle through the two-heptad repeat domain. To develop effective vaccines and therapeutics against COVID-19, it is critical to express and purify the SARS-CoV-2 Spike protein. Extensive studies have been conducted on expression of a complete recombinant spike protein or its fragments. This review provides an in-depth analysis of the different expression systems employed for spike protein expression, along with their advantages and disadvantages.

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来源期刊

Protein expression and purification 生物-生化研究方法

CiteScore

3.70

自引率

6.20%

发文量

120

审稿时长

32 days

期刊介绍： Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.