Triticum durum-Haynaldia villosa 两性杂交种杂交坏死的表型特征和基因图谱。

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-07-15 DOI:10.1007/s00122-024-04691-0
Yangqi Liu, Jinhong Liu, Zhenpu Huang, Kaiwen Fan, Xinshuo Guo, Liping Xing, Aizhong Cao
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摘要

关键信息对Haynaldia villosa的杂交坏死基因进行了表型、生理和遗传鉴定,并将相关基因Ne-V绘制到染色体臂2VL上。通过广泛杂交将野生近缘种的遗传变异引入普通小麦是丰富遗传多样性和促进小麦育种的重要策略。然而,种间杂交或种内杂交的后代往往会出现杂交坏死(一种遗传自体免疫综合征),从而限制了杂交亲本的选择和有益基因的金字塔化。为了利用Haynaldia villosa(2n = 2x = 14,VV)的种质资源,我们在硬质小麦(2n = 4x = 28,AABB)和多个H. villosa登录品之间进行了广泛杂交,合成了两倍体(2n = 6x = 42,AABBVV)。这项研究发现,上述杂交产生的两倍体中有 61.5%出现杂交坏死,有些两倍体甚至在成熟前就已经死亡。然而,不同后代发生坏死的时间和严重程度差异很大,这表明在 H. villosa 品种中存在多个遗传位点或同一遗传位点中的多个等位基因导致杂交坏死。在叶片正常的两倍体 STH59-1 和叶片坏死的两倍体 STH59-2 之间构建的 F2 和衍生的 F2:3 群体中进行了遗传分析。一个半显性杂交坏死相关基因 Ne-V 被映射到 2V 染色体长臂上 11.8 厘米的遗传间隔上,这是在小麦相关物种中发现的一个新的遗传位点。此外,杂交种坏死与 H2O2 积累和细胞死亡的增强有关,并受温度和光照的影响。我们的研究结果为克隆 Ne-V 基因和探索其分子机制奠定了基础。
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Phenotypic characterization and gene mapping of hybrid necrosis in Triticum durum-Haynaldia villosa amphiploids.

Key message: Phenotypical, physiological and genetic characterization was carried out on the hybrid necrosis gene from Haynaldia villosa, and the related gene Ne-V was mapped to chromosome arm 2VL. Introducing genetic variation from wild relatives into common wheat through wide crosses is a vital strategy for enriching genetic diversity and promoting wheat breeding. However, hybrid necrosis, a genetic autoimmunity syndrome, often occurs in the offspring of interspecific or intraspecific crosses, restricting both the selection of hybrid parents and the pyramiding of beneficial genes. To utilize the germplasms of Haynaldia villosa (2n = 2x = 14, VV), we conducted wide hybridization between durum wheat (2n = 4x = 28, AABB) and multiple H. villosa accessions to synthesize the amphiploids (2n = 6x = 42, AABBVV). This study revealed that 61.5% of amphiploids derived from the above crosses exhibited hybrid necrosis, with some amphiploids even dying before reaching maturity. However, the initiation time and severity of necrosis varied dramatically among the progenies, suggesting that there were multiple genetic loci or multiple alleles in the same genetic locus conferring to hybrid necrosis in H. villosa accessions. Genetic analysis was performed on the F2 and derived F2:3 populations, which were constructed between amphiploid STH59-1 with normal leaves and amphiploid STH59-2 with necrotic leaves. A semidominant hybrid necrosis-related gene, Ne-V, was mapped to an 11.8-cM genetic interval on the long arm of chromosome 2V, representing a novel genetic locus identified in Triticum-related species. In addition, the hybrid necrosis was correlated with enhanced H2O2 accumulation and cell death, and it was influenced by the temperature and light. Our findings provide a foundation for cloning the Ne-V gene and exploring its molecular mechanism.

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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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