噬菌体和鼠疫耶尔森氏菌的共生以及Marmota himalayana鼠疫的特征。

IF 3.9 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied and Environmental Microbiology Pub Date : 2024-08-21 Epub Date: 2024-07-18 DOI:10.1128/aem.00995-24
Dongyue Lyu, Qun Duan, Ran Duan, Shuai Qin, Xiaojin Zheng, Xinmin Lu, Asaiti Bukai, Peng Zhang, Haonan Han, Zhaokai He, Hanyu Sha, Di Wu, Meng Xiao, Huaiqi Jing, Xin Wang
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引用次数: 0

摘要

2020 年至 2023 年,在青藏高原旱獭鼠疫重点地区开展了动物鼠疫监测。活体捕获的旱獭血清F1抗体阳性率为22.89%,从旱獭尸体中分离出的鼠疫耶尔森氏菌感染率为43.40%。感染鼠疫的旱獭尸体的脾脏症状指数明显较高(P < 0.05)。从旱獭尸体骨髓中分离出21个鼠疫噬菌体,其中一个鼠疫噬菌体(AKS2022HT87GU_phi)与鼠疫噬菌体共生。显微镜检查发现,鼠疫 Y. AKS2022HT87 的裂解菌落和非裂解菌落共存。全基因组分析表明,Y. pestis AKS2022HT87的某些菌株携带与噬菌体AKS2022HT87GU_phi一致的噬菌体DNA片段。重要意义噬菌体和宿主细菌通常在体内或土壤环境中通过复杂和相互依存的微生物相互作用共存。然而,由于培养基养分有限,在体外重现这种共生状态仍然具有挑战性。在这项研究中,我们在一个Marmota himalayana标本中发现了鼠疫耶尔森菌和特定噬菌体之间的天然共生关系。流行病学分析显示了鼠疫疫情严重地区鼠疫耶尔森菌和特定噬菌体的特征。最重要的是,比较基因组学分析了鼠疫酵母菌和噬菌体在不同时期的基因变化,揭示了它们共生关系的动态和演变性质。这些都是研究共生机制的关键步骤。
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Symbiosis of a lytic bacteriophage and Yersinia pestis and characteristics of plague in Marmota himalayana.

Surveillance for animal plague was conducted in the Marmota himalayana plague focus of the Qinghai-Tibet Plateau from 2020 to 2023. A 22.89% positive rate of serum F1 antibody was detected in live-caught marmots, alongside a 43.40% incidence of Yersinia pestis isolation from marmot carcasses. Marmot carcasses infected with plague exhibited a significantly higher spleen-somatic index (P < 0.05). Twenty-one Y. pestis-specific phages were isolated, among which one Y. pestis lytic phage (AKS2022HT87GU_phi) was isolated from the bone marrow of a marmot carcass (no. AKS2022HT87) and was found to be symbiotic with Y. pestis. Microscopy revealed the coexistence of lysed and non-lysed colonies of Y. pestis AKS2022HT87. Genome-wide analysis showed that certain strains of the Y. pestis AKS2022HT87 carried phage DNA fragments consistent with phage AKS2022HT87GU_phi. The rare symbiotic relationship between a lytic phage and Y. pestis observed in vitro was highlighted in this study, laying the basis for further exploring the relationship between Y. pestis and its bacteriophages.IMPORTANCEBacteriophages and host bacteria commonly coexist in vivo or in soil environments through complex and interdependent microbial interactions. However, recapitulating this symbiotic state remains challenging in vitro due to limited medium nutrients. In this work, the natural symbiosis between Yersinia pestis and specific phages has been discovered in a Marmota himalayana specimen. Epidemiological analysis presented the characteristics of the Y. pestis and specific phages in the area with a strong plague epidemic. Crucially, comparative genomics has been conducted to analyze the genetic changes in both the Y. pestis and phages over different periods, revealing the dynamic and evolving nature of their symbiosis. These are the critical steps to study the mechanism of the symbiosis.

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来源期刊
Applied and Environmental Microbiology
Applied and Environmental Microbiology 生物-生物工程与应用微生物
CiteScore
7.70
自引率
2.30%
发文量
730
审稿时长
1.9 months
期刊介绍: Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.
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