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引用次数: 0
摘要
染色质状态在塑造细胞整体状态和命运方面发挥着关键作用。要全面了解细胞中染色质的功能状态,需要同时检测多个不同的生化方面。这些方面包括 DNA 甲基化、染色质可及性、染色体构象、组蛋白翻译后修饰等。虽然这无疑是一项具有挑战性的任务,但在过去几年中,许多新的创造性方法已经开发出来,现在可以在单细胞分辨率下对染色质的这些不同方面进行联合检测。这一领域正进入一个令人兴奋的阶段,技术的改进、测序成本的降低和计算的创新为剖析组织中染色质状态的多样性以及这些状态如何影响基因调控提供了新的机遇。在这篇综述中,我将讨论当前多因素染色质图谱分析的各种实验方法,强调一些实验和分析方面的挑战,以及一些有待进一步创新的领域。
Progress in multifactorial single-cell chromatin profiling methods.
Chromatin states play a key role in shaping overall cellular states and fates. Building a complete picture of the functional state of chromatin in cells requires the co-detection of several distinct biochemical aspects. These span DNA methylation, chromatin accessibility, chromosomal conformation, histone posttranslational modifications, and more. While this certainly presents a challenging task, over the past few years many new and creative methods have been developed that now enable co-assay of these different aspects of chromatin at single cell resolution. This field is entering an exciting phase, where a confluence of technological improvements, decreased sequencing costs, and computational innovation are presenting new opportunities to dissect the diversity of chromatin states present in tissues, and how these states may influence gene regulation. In this review, I discuss the spectrum of current experimental approaches for multifactorial chromatin profiling, highlight some of the experimental and analytical challenges, as well as some areas for further innovation.
期刊介绍:
Biochemical Society Transactions is the reviews journal of the Biochemical Society. Publishing concise reviews written by experts in the field, providing a timely snapshot of the latest developments across all areas of the molecular and cellular biosciences.
Elevating our authors’ ideas and expertise, each review includes a perspectives section where authors offer comment on the latest advances, a glimpse of future challenges and highlighting the importance of associated research areas in far broader contexts.