用于电化学检测基孔肯雅病毒的基于银纳米粒子修饰的柔性碳墨印刷电极的适配体

Biosensors Pub Date : 2024-07-16 DOI:10.3390/bios14070344
Pradakshina Sharma, Mohd. Rahil Hasan, Ubaid Mushtaq Naikoo, Shaheen Khatoon, R. Pilloton, Jagriti Narang
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引用次数: 0

摘要

医疗设备已从最初的笨重形态发展为智能设备。然而,它们的刚性阻碍了它们与日常生活的无缝融合。可拉伸、纺织品和柔性电子领域是新兴的研究领域,有可能推动重大技术进步。本研究介绍了一种基于实验室的技术,用于生产检测基孔肯雅病毒的高灵敏度柔性生物传感器。这些生物传感器基于 0D 纳米材料,展示了伏安法的重大进展。电化学平台是利用模板印刷(StPE)技术制作的。调整生物传感器的设置涉及选择与银纳米粒子(AgNPs)结合的适配体作为生物识别元素。该生物传感器被用于在含有 0.5 mM 铁氰化钾(氧化还原对)的溶液中对基孔肯雅病毒抗原(CHIKV-Ag)进行伏安识别。该生物传感器用于评估人体血清样本中的 CHIKV-Ag。它的线性检测范围为 0.1 ng/mL 至 1 μg/mL,CHIKV-Ag 的检测限为 0.1 ng/mL。由于其生产的灵活性和零维纳米结构所显示的电催化特性,所提出的方法为基于贴体的生物电子学提供了具有成本效益和量身定制的创新机会,从而拓宽了这一领域的范围。
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Aptamer Based on Silver Nanoparticle-Modified Flexible Carbon Ink Printed Electrode for the Electrochemical Detection of Chikungunya Virus
Medical devices have progressed from their initial bulky forms to smart devices. However, their rigidity hampers their seamless integration into everyday life. The fields of stretchable, textile, and flexible electronics are emerging research areas with the potential to drive significant technological progress. This research presents a laboratory-based technique to produce highly sensitive and flexible biosensors for detecting the chikungunya virus. These biosensors are based on 0D nanomaterials and demonstrate significant advancements in voltammetry. The electrochemical platform was created utilizing the stencil printing (StPE) technique. Adapting the biosensor setup involved the selection of aptamer as the biorecognition element bound with silver nanoparticles (AgNPs). This biosensor was employed in the voltammetric identification of the Chikungunya virus antigen (CHIKV-Ag) within a solution containing 0.5 mM potassium ferro/ferri cyanide, a redox pair. The biosensor was employed to evaluate CHIKV-Ag within a human serum sample. It demonstrated a linear detection span ranging from 0.1 ng/mL to 1 μg/mL, with a detection limit of 0.1 ng/mL for CHIKV-Ag. The proposed approach, due to its flexibility in production and the electrocatalytic attributes displayed by the zero-dimensional nanostructure, presents innovative opportunities for cost-effective and tailored aptamer-based bioelectronics, thereby broadening the scope of this domain.
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