挖掘葡萄牙特有的 Santolina impressa 的生物活性潜力并探索其新的应用领域

Plants Pub Date : 2024-07-15 DOI:10.3390/plants13141943
J. Alves-Silva, Sónia Pedreiro, M. Zuzarte, Maria Teresa Cruz, A. Figueirinha, Lígia Salgueiro
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引用次数: 0

摘要

Santolina impressa 是葡萄牙特有的一种植物,传统上用于治疗各种感染和疾病。本研究旨在通过 HPLC-DAD-ESI-MSn 评估其化学成分,并验证其抗炎潜力。此外,研究还揭示了其抗氧化能力以及对伤口愈合、脂肪生成、黑色素生成和细胞衰老的影响,所有这些过程中,失调的炎症反应都起着关键作用。在脂多糖(LPS)刺激的巨噬细胞中评估了抗炎潜力,使用划痕伤口试验测定了细胞迁移,在 T0901317 刺激的角质细胞中评估了脂肪生成,在 3-异丁基-1-甲基黄嘌呤(IBMX)激活的黑色素细胞中评估了黑色素生成。依托泊苷用于诱导成纤维细胞衰老。我们的研究结果表明,其化学成分主要以二咖啡酰奎宁酸和少量黄酮醇为特征。关于输液的生物活性潜力,通过降低一氧化氮的产生、诱导型一氧化氮合酶和前白细胞介素-1β蛋白的水平,抗炎效果显而易见。此外,还观察到成纤维细胞迁移的减少,以及细胞内脂质积累和黑色素生成的抑制。此外,输注还能降低衰老相关的β-半乳糖苷酶活性、γH2AX 核积累以及 p53 和 p21 蛋白水平。总之,这项研究证实了 S. impressa 的传统用途,并赋予了其在制药和皮肤美容行业中的其他特性。
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Unlocking the Bioactive Potential and Exploring Novel Applications for Portuguese Endemic Santolina impressa
The infusion of Santolina impressa, an endemic Portuguese plant, is traditionally used to treat various infections and disorders. This study aimed to assess its chemical profile by HPLC-DAD-ESI-MSn and validate its anti-inflammatory potential. In addition, the antioxidant capacity and effects on wound healing, lipogenesis, melanogenesis, and cellular senescence, all processes in which a dysregulated inflammatory response plays a pivotal role, were unveiled. The anti-inflammatory potential was assessed in lipopolysaccharide (LPS)-stimulated macrophages, cell migration was determined using a scratch wound assay, lipogenesis was assessed on T0901317-stimulated keratinocytes and melanogenesis on 3-isobutyl-1-methylxanthine (IBMX)-activated melanocytes. Etoposide was used to induce senescence in fibroblasts. Our results point out a chemical composition predominantly characterized by dicaffeoylquinic acids and low amounts of flavonols. Regarding the infusion’s bioactive potential, an anti-inflammatory effect was evident through a decrease in nitric oxide production and inducible nitric oxide synthase and pro-interleukin-1β protein levels. Moreover, a decrease in fibroblast migration was observed, as well as an inhibition in both intracellular lipid accumulation and melanogenesis. Furthermore, the infusion decreased senescence-associated β-galactosidase activity, γH2AX nuclear accumulation and both p53 and p21 protein levels. Overall, this study confirms the traditional uses of S. impressa and ascribes additional properties of interest in the pharmaceutical and dermocosmetics industries.
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