{"title":"通过形态学、核型和 COI 基因序列证实了 Chironomus nuditarsis Keyl, 1961 年在亚美尼亚塞万湖的首次记录","authors":"V. Bolshakov, A. Prokin, E. Ivanova, E. Movergoz","doi":"10.3897/compcytogen.18.126130","DOIUrl":null,"url":null,"abstract":"Chironomus nuditarsis Keyl, 1961 is recorded from Sevan Lake for the first time. This species is widespread in Europe, the Caucasus, and Siberia. For species identification, we used a comprehensive approach that included morphological, cytogenetic and molecular genetic analyses. Morphological analysis showed a high similarity with the description. Nine chromosome banding sequences ndtA1, ndtA2, ndtB2, ndtC1, ndtD1, ndtE1, ndtF1, ndtG1, and ndtG2 were found. The banding sequences ndtA1, ndtA2, ndtG1, and ndtG2 are species-specific for C. nuditarsis and allow us to accurately distinguish it from the sibling species Ch. curabilis Belyanina, Sigareva et Loginova, 1990. Molecular-genetic analysis of the COI gene sequences has shown low genetic distances of 0.38–0.95% in the sibling species Ch. nuditarsis and Ch. curabilis complex and the insufficiency of using a single COI as a molecular marker for their separation.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":"50 3","pages":""},"PeriodicalIF":18.0000,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The first record of Chironomus nuditarsis Keyl, 1961 from Sevan Lake (Armenia) confirmed by morphology, karyotype and COI gene sequence\",\"authors\":\"V. Bolshakov, A. Prokin, E. Ivanova, E. Movergoz\",\"doi\":\"10.3897/compcytogen.18.126130\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Chironomus nuditarsis Keyl, 1961 is recorded from Sevan Lake for the first time. This species is widespread in Europe, the Caucasus, and Siberia. For species identification, we used a comprehensive approach that included morphological, cytogenetic and molecular genetic analyses. Morphological analysis showed a high similarity with the description. Nine chromosome banding sequences ndtA1, ndtA2, ndtB2, ndtC1, ndtD1, ndtE1, ndtF1, ndtG1, and ndtG2 were found. The banding sequences ndtA1, ndtA2, ndtG1, and ndtG2 are species-specific for C. nuditarsis and allow us to accurately distinguish it from the sibling species Ch. curabilis Belyanina, Sigareva et Loginova, 1990. Molecular-genetic analysis of the COI gene sequences has shown low genetic distances of 0.38–0.95% in the sibling species Ch. nuditarsis and Ch. curabilis complex and the insufficiency of using a single COI as a molecular marker for their separation.\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":\"50 3\",\"pages\":\"\"},\"PeriodicalIF\":18.0000,\"publicationDate\":\"2024-07-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3897/compcytogen.18.126130\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3897/compcytogen.18.126130","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
Chironomus nuditarsis Keyl, 1961 年首次在塞万湖记录到。该物种广泛分布于欧洲、高加索地区和西伯利亚。为了鉴定该物种，我们采用了包括形态学、细胞遗传学和分子遗传学分析在内的综合方法。形态学分析表明，该物种与描述高度相似。发现了九条染色体带状序列ndtA1、ndtA2、ndtB2、ndtC1、ndtD1、ndtE1、ndtF1、ndtG1和ndtG2。带状序列 ndtA1、ndtA2、ndtG1 和 ndtG2 是 C. nuditarsis 的物种特异性序列，使我们能够准确地将其与 Ch. curabilis Belyanina, Sigareva et Loginova, 1990 的同胞种区分开来。对 COI 基因序列的分子遗传分析表明，裸冠菊 Ch. nuditarsis 和樱草菊 Ch. curabilis 复合物的遗传距离很低，只有 0.38-0.95% 的遗传距离，因此不能用单一 COI 作为分子标记来区分它们。
The first record of Chironomus nuditarsis Keyl, 1961 from Sevan Lake (Armenia) confirmed by morphology, karyotype and COI gene sequence
Chironomus nuditarsis Keyl, 1961 is recorded from Sevan Lake for the first time. This species is widespread in Europe, the Caucasus, and Siberia. For species identification, we used a comprehensive approach that included morphological, cytogenetic and molecular genetic analyses. Morphological analysis showed a high similarity with the description. Nine chromosome banding sequences ndtA1, ndtA2, ndtB2, ndtC1, ndtD1, ndtE1, ndtF1, ndtG1, and ndtG2 were found. The banding sequences ndtA1, ndtA2, ndtG1, and ndtG2 are species-specific for C. nuditarsis and allow us to accurately distinguish it from the sibling species Ch. curabilis Belyanina, Sigareva et Loginova, 1990. Molecular-genetic analysis of the COI gene sequences has shown low genetic distances of 0.38–0.95% in the sibling species Ch. nuditarsis and Ch. curabilis complex and the insufficiency of using a single COI as a molecular marker for their separation.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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