银-壳聚糖纳米复合材料对水生微型甲壳动物大型蚤和板鳃蚤以及天然发光细菌弧菌的毒性

Nanomaterials Pub Date : 2024-07-12 DOI:10.3390/nano14141193
M. Sihtmäe, Jüri Laanoja, I. Blinova, A. Kahru, K. Kasemets
{"title":"银-壳聚糖纳米复合材料对水生微型甲壳动物大型蚤和板鳃蚤以及天然发光细菌弧菌的毒性","authors":"M. Sihtmäe, Jüri Laanoja, I. Blinova, A. Kahru, K. Kasemets","doi":"10.3390/nano14141193","DOIUrl":null,"url":null,"abstract":"All novel materials should be analyzed for their potential environmental hazard. In this study, the toxicity of different silver–chitosan nanocomposites—potential candidates for wound dressings or antimicrobial surface coatings—was evaluated using environmentally relevant aquatic microcrustaceans Daphnia magna and Thamnocephalus platyurus and naturally luminescent bacteria Vibrio fischeri. Three silver-chitosan nanocomposites (nAgCSs) with different weight ratios of Ag to CS were studied. Citrate-coated silver nanoparticles (nAg-Cit), AgNO3 (ionic control) and low molecular weight chitosan (LMW CS) were evaluated in parallel. The primary size of nAgCSs was ~50 nm. The average hydrodynamic sizes in deionized water were ≤100 nm, and the zeta potential values were positive (16–26 mV). The nAgCSs proved very toxic to aquatic crustaceans: the 48-h EC50 value for D. magna was 0.065–0.232 mg/L, and the 24-h LC50 value for T. platyurus was 0.25–1.04 mg/L. The toxic effect correlated with the shedding of Ag ions (about 1%) from nAgCSs. Upon exposure of V. fischeri to nAgCSs for 30 min, bacterial luminescence was inhibited by 50% at 13–33 mg/L. However, the inhibitory effect (minimum bactericidal concentration, MBC) on bacterial growth upon 1 h exposure was observed at higher concentrations of nAgCSs, 40–65 mg/L. LMW CS inhibited bacterial luminescence upon 30-min exposure at 5.6 mg/L, but bacterial growth was inhibited at a much higher concentration (1 h MBC > 100 mg/L). The multi-trophic test battery, where D. magna was the most sensitive test organism, ranked the silver-chitosan nanocomposites from ‘extremely toxic’ [L(E)C50 ≤ 0.1 mg/L] to ‘very toxic’ [L(E)C50 > 0.1–1 mg/L]. Chitosan was toxic (EC(L)50) to crustaceans at ~12 mg/L, and ranked accordingly as ‘harmful’ [L(E)C50 > 10–100 mg/L]. Thus, silver-chitosan nanocomposites may pose a hazard to aquatic organisms and must be handled accordingly.","PeriodicalId":508599,"journal":{"name":"Nanomaterials","volume":"9 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Toxicity of Silver–Chitosan Nanocomposites to Aquatic Microcrustaceans Daphnia magna and Thamnocephalus platyurus and Naturally Luminescent Bacteria Vibrio fischeri\",\"authors\":\"M. Sihtmäe, Jüri Laanoja, I. Blinova, A. Kahru, K. Kasemets\",\"doi\":\"10.3390/nano14141193\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"All novel materials should be analyzed for their potential environmental hazard. In this study, the toxicity of different silver–chitosan nanocomposites—potential candidates for wound dressings or antimicrobial surface coatings—was evaluated using environmentally relevant aquatic microcrustaceans Daphnia magna and Thamnocephalus platyurus and naturally luminescent bacteria Vibrio fischeri. Three silver-chitosan nanocomposites (nAgCSs) with different weight ratios of Ag to CS were studied. Citrate-coated silver nanoparticles (nAg-Cit), AgNO3 (ionic control) and low molecular weight chitosan (LMW CS) were evaluated in parallel. The primary size of nAgCSs was ~50 nm. The average hydrodynamic sizes in deionized water were ≤100 nm, and the zeta potential values were positive (16–26 mV). The nAgCSs proved very toxic to aquatic crustaceans: the 48-h EC50 value for D. magna was 0.065–0.232 mg/L, and the 24-h LC50 value for T. platyurus was 0.25–1.04 mg/L. The toxic effect correlated with the shedding of Ag ions (about 1%) from nAgCSs. Upon exposure of V. fischeri to nAgCSs for 30 min, bacterial luminescence was inhibited by 50% at 13–33 mg/L. However, the inhibitory effect (minimum bactericidal concentration, MBC) on bacterial growth upon 1 h exposure was observed at higher concentrations of nAgCSs, 40–65 mg/L. LMW CS inhibited bacterial luminescence upon 30-min exposure at 5.6 mg/L, but bacterial growth was inhibited at a much higher concentration (1 h MBC > 100 mg/L). The multi-trophic test battery, where D. magna was the most sensitive test organism, ranked the silver-chitosan nanocomposites from ‘extremely toxic’ [L(E)C50 ≤ 0.1 mg/L] to ‘very toxic’ [L(E)C50 > 0.1–1 mg/L]. Chitosan was toxic (EC(L)50) to crustaceans at ~12 mg/L, and ranked accordingly as ‘harmful’ [L(E)C50 > 10–100 mg/L]. Thus, silver-chitosan nanocomposites may pose a hazard to aquatic organisms and must be handled accordingly.\",\"PeriodicalId\":508599,\"journal\":{\"name\":\"Nanomaterials\",\"volume\":\"9 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomaterials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/nano14141193\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/nano14141193","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

所有新型材料都应分析其潜在的环境危害。本研究利用与环境相关的水生微型甲壳类动物大型蚤(Daphnia magna)和板尾蚤(Thamnocephalus platyurus)以及天然发光细菌弧菌(Vibrio fischeri)评估了不同银-壳聚糖纳米复合材料的毒性。研究了三种不同重量比的银-壳聚糖纳米复合材料(nAgCSs)。同时评估了柠檬酸银纳米粒子(nAg-Cit)、AgNO3(离子对照)和低分子量壳聚糖(LMW CS)。nAgCSs 的主要尺寸为 ~50 nm。去离子水中的平均水动力尺寸≤100 nm,zeta 电位值为正(16-26 mV)。nAgCSs 对水生甲壳类动物有剧毒:对 D. magna 的 48 小时 EC50 值为 0.065-0.232 mg/L,对 T. platyurus 的 24 小时 LC50 值为 0.25-1.04 mg/L。毒性效应与 nAgCSs 中脱落的银离子(约 1%)有关。将 V. fischeri 暴露于 nAgCSs 30 分钟后,当浓度为 13-33 mg/L 时,细菌发光受到 50%的抑制。然而,在较高浓度的 nAgCSs(40-65 毫克/升)条件下,接触 1 小时后细菌生长的抑制作用(最低杀菌浓度,MBC)才会显现。低分子量 CS 在 5.6 毫克/升的浓度下暴露 30 分钟后可抑制细菌发光,但在更高浓度下(1 小时的 MBC > 100 毫克/升)细菌生长受到抑制。在以大型蚤为最敏感测试生物的多营养测试中,银-壳聚糖纳米复合材料的毒性从 "剧毒"[L(E)C50 ≤ 0.1 mg/L]到 "极毒"[L(E)C50 > 0.1-1 mg/L]不等。壳聚糖对甲壳类动物的毒性(EC(L)50)约为 12 毫克/升,因此被列为 "有害"[L(E)C50 > 10-100 毫克/升]。因此,银-壳聚糖纳米复合材料可能会对水生生物造成危害,必须进行相应处理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Toxicity of Silver–Chitosan Nanocomposites to Aquatic Microcrustaceans Daphnia magna and Thamnocephalus platyurus and Naturally Luminescent Bacteria Vibrio fischeri
All novel materials should be analyzed for their potential environmental hazard. In this study, the toxicity of different silver–chitosan nanocomposites—potential candidates for wound dressings or antimicrobial surface coatings—was evaluated using environmentally relevant aquatic microcrustaceans Daphnia magna and Thamnocephalus platyurus and naturally luminescent bacteria Vibrio fischeri. Three silver-chitosan nanocomposites (nAgCSs) with different weight ratios of Ag to CS were studied. Citrate-coated silver nanoparticles (nAg-Cit), AgNO3 (ionic control) and low molecular weight chitosan (LMW CS) were evaluated in parallel. The primary size of nAgCSs was ~50 nm. The average hydrodynamic sizes in deionized water were ≤100 nm, and the zeta potential values were positive (16–26 mV). The nAgCSs proved very toxic to aquatic crustaceans: the 48-h EC50 value for D. magna was 0.065–0.232 mg/L, and the 24-h LC50 value for T. platyurus was 0.25–1.04 mg/L. The toxic effect correlated with the shedding of Ag ions (about 1%) from nAgCSs. Upon exposure of V. fischeri to nAgCSs for 30 min, bacterial luminescence was inhibited by 50% at 13–33 mg/L. However, the inhibitory effect (minimum bactericidal concentration, MBC) on bacterial growth upon 1 h exposure was observed at higher concentrations of nAgCSs, 40–65 mg/L. LMW CS inhibited bacterial luminescence upon 30-min exposure at 5.6 mg/L, but bacterial growth was inhibited at a much higher concentration (1 h MBC > 100 mg/L). The multi-trophic test battery, where D. magna was the most sensitive test organism, ranked the silver-chitosan nanocomposites from ‘extremely toxic’ [L(E)C50 ≤ 0.1 mg/L] to ‘very toxic’ [L(E)C50 > 0.1–1 mg/L]. Chitosan was toxic (EC(L)50) to crustaceans at ~12 mg/L, and ranked accordingly as ‘harmful’ [L(E)C50 > 10–100 mg/L]. Thus, silver-chitosan nanocomposites may pose a hazard to aquatic organisms and must be handled accordingly.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Advancing Silver Bismuth Sulfide Quantum Dots for Practical Solar Cell Applications Anisotropic SmFe10V2 Bulk Magnets with Enhanced Coercivity via Ball Milling Process A Novel Fabrication of Hematite Nanoparticles via Recycling of Titanium Slag by Pyrite Reduction Technology Plant-Derived Extracellular Vesicles as a Novel Frontier in Cancer Therapeutics Biological Nano-Agrochemicals for Crop Production as an Emerging Way to Address Heat and Associated Stresses
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1