Tayyebe Tavakoli, Leila Mohammad Taghizadeh kashani, Meysam Shirzad, Mohammad Reza Memarzadeh, Ali Shamsizadeh, Ayat Kaeidi, Jalal Hassanshahi, Mahsa Hassanipour
{"title":"传统草药配方(Azaraghi Majoon)对雄性大鼠鸦片使用障碍的影响","authors":"Tayyebe Tavakoli, Leila Mohammad Taghizadeh kashani, Meysam Shirzad, Mohammad Reza Memarzadeh, Ali Shamsizadeh, Ayat Kaeidi, Jalal Hassanshahi, Mahsa Hassanipour","doi":"10.1007/s13596-024-00771-0","DOIUrl":null,"url":null,"abstract":"<div><p>Opium Use Disorder (OUD) has the highest rate of opioid use disorder in Iran. The present study was designed to investigate the effect of Azaraghi Majoon (AM), a traditional Persian compound, on a rat model of OUD. AM was prepared from a combination of 14 herbs plus honey, standardized by strychnine and brucine using high-performance liquid chromatography, and administered by gavage. One hundred twenty male mice were used to investigate the effect of AM on the manifestations of opium withdrawal syndrome and craving for opium. Naloxone-precipitated withdrawal signs and conditioned place preference (CPP) test were used to assess the scopes mentioned above of OUD, respectively. A modified schedule of opium dependence was used to assess physical dependence (last for eight days). 50 mg/ml/kg intraperitoneal opium and 50, 100, or 150 mg/ml/kg gavaged AM in different groups were administered in the CPP paradigm. In the CPP test, treating opium-addicted animals with AM (50 and 150 mg/kg for time and 50, 100, and 150 mg/kg for frequency) resulted in the extinction of preference for drug compartments. Also, the administration of AM decreased the number of jumping, diarrhea, rearing, and grooming following naloxone-precipitated opium withdrawal signs. As AM could improve both physical and psychological withdrawal signs of opium use disorder in rats in this study, the authors suggest consideration of clinical trials to investigate possible beneficial effects of AM in OUD patients.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of a traditional herbal formulation (Azaraghi Majoon) on opium use disorder in male rats\",\"authors\":\"Tayyebe Tavakoli, Leila Mohammad Taghizadeh kashani, Meysam Shirzad, Mohammad Reza Memarzadeh, Ali Shamsizadeh, Ayat Kaeidi, Jalal Hassanshahi, Mahsa Hassanipour\",\"doi\":\"10.1007/s13596-024-00771-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Opium Use Disorder (OUD) has the highest rate of opioid use disorder in Iran. The present study was designed to investigate the effect of Azaraghi Majoon (AM), a traditional Persian compound, on a rat model of OUD. AM was prepared from a combination of 14 herbs plus honey, standardized by strychnine and brucine using high-performance liquid chromatography, and administered by gavage. One hundred twenty male mice were used to investigate the effect of AM on the manifestations of opium withdrawal syndrome and craving for opium. Naloxone-precipitated withdrawal signs and conditioned place preference (CPP) test were used to assess the scopes mentioned above of OUD, respectively. A modified schedule of opium dependence was used to assess physical dependence (last for eight days). 50 mg/ml/kg intraperitoneal opium and 50, 100, or 150 mg/ml/kg gavaged AM in different groups were administered in the CPP paradigm. In the CPP test, treating opium-addicted animals with AM (50 and 150 mg/kg for time and 50, 100, and 150 mg/kg for frequency) resulted in the extinction of preference for drug compartments. Also, the administration of AM decreased the number of jumping, diarrhea, rearing, and grooming following naloxone-precipitated opium withdrawal signs. As AM could improve both physical and psychological withdrawal signs of opium use disorder in rats in this study, the authors suggest consideration of clinical trials to investigate possible beneficial effects of AM in OUD patients.</p></div>\",\"PeriodicalId\":7613,\"journal\":{\"name\":\"Advances in Traditional Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-07-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Traditional Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13596-024-00771-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-024-00771-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Effect of a traditional herbal formulation (Azaraghi Majoon) on opium use disorder in male rats
Opium Use Disorder (OUD) has the highest rate of opioid use disorder in Iran. The present study was designed to investigate the effect of Azaraghi Majoon (AM), a traditional Persian compound, on a rat model of OUD. AM was prepared from a combination of 14 herbs plus honey, standardized by strychnine and brucine using high-performance liquid chromatography, and administered by gavage. One hundred twenty male mice were used to investigate the effect of AM on the manifestations of opium withdrawal syndrome and craving for opium. Naloxone-precipitated withdrawal signs and conditioned place preference (CPP) test were used to assess the scopes mentioned above of OUD, respectively. A modified schedule of opium dependence was used to assess physical dependence (last for eight days). 50 mg/ml/kg intraperitoneal opium and 50, 100, or 150 mg/ml/kg gavaged AM in different groups were administered in the CPP paradigm. In the CPP test, treating opium-addicted animals with AM (50 and 150 mg/kg for time and 50, 100, and 150 mg/kg for frequency) resulted in the extinction of preference for drug compartments. Also, the administration of AM decreased the number of jumping, diarrhea, rearing, and grooming following naloxone-precipitated opium withdrawal signs. As AM could improve both physical and psychological withdrawal signs of opium use disorder in rats in this study, the authors suggest consideration of clinical trials to investigate possible beneficial effects of AM in OUD patients.
期刊介绍:
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.