D. Dzhalilova, M. V. Silina, N. Zolotova, T. S. Portnova, M. D. Vagabov, I. Tsvetkov, O. Makarova
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引用次数: 0
摘要
在高耐受性(HR)和低耐受性(LR)的成年雄性 C57BL/6 小鼠中,在腹腔注射偶氮甲烷并随后服用 3 个周期的葡聚糖硫酸钠 70 天后,对结肠肿瘤的形态特征和血液参数进行了评估。宏观分析显示,35%的HR动物(20只动物中的7只)和31%的LR动物(13只动物中的4只)在结肠远端发现了肿瘤。远端结肠的显微镜分析显示,75% 的 HR 小鼠(20 只中的 15 只)和 69% 的 LR 小鼠(13 只中的 9 只)患有肿瘤。肿瘤表现为腺体上皮内瘤变和腺癌;HR 组和 LR 组的肿瘤发生率和面积没有差异。在肿瘤区域的远端结肠粘膜中,神经内分泌细胞和鹅口疮细胞的数量在对比组中相似。然而,与相应的对照组相比,实验组的 HR 和 LR 小鼠肿瘤中的鹅口疮细胞含量较低,内分泌细胞含量较高。在外周血中,实验组的 HR 小鼠和 LR 小鼠的红细胞计数和血红蛋白含量均下降;只有 HR 小鼠的单核细胞相对数量增加,而 LR 小鼠的淋巴细胞和单核细胞绝对数量减少。因此,在服用偶氮甲烷和葡聚糖硫酸钠 70 天后,小鼠的肿瘤表现为腺上皮内瘤变和腺癌,其发生率和面积在对缺氧耐受性不同的动物之间没有差异。
Morphological Characteristics of Colon Tumors in Mice with Different Tolerance to Hypoxia.
In adult male C57BL/6 mice with high (HR) and low (LR) resistance to hypoxia, morphological features of colon tumors and blood parameters were evaluated 70 days after intraperitoneal injection of azoxymethane and subsequent consumption of 3 cycles of dextran sulfate sodium. On macroscopic analysis, tumors were found in the distal colon in 35% (7 of 20 animals) of HR and 31% (4 of 13 animals) of LR animals. Microscopic analysis of the distal colon revealed tumors in 75% (15 of 20 animals) of HR and 69% (9 of 13 animals) of LR mice. The tumors were presented by areas of glandular intraepithelial neoplasia and adenocarcinomas; the incidence and the area of the tumors did not differ in groups of HR and LR mice. The number of neuroendocrine and goblet cells in the distal colon mucosa in the areas of tumors was similar in the compared groups. However, in both HR and LR mice of the experimental groups, the content of goblet cells in tumors was lower and the content of endocrine cells was higher than in the corresponding control groups. In the peripheral blood, the erythrocyte count and hemoglobin content decreased in HR and LR mice of the experimental groups; the relative number of monocytes increased only in HR mice and the absolute number of lymphocytes and monocytes decreased in LR mice. Thus, 70 days after azoxymethane administration and dextran sulfate sodium consumption, the tumors in mice were presented by glandular intraepithelial neoplasia and adenocarcinomas, and their incidence and area did not differ between animals with different tolerance to hypoxia.
期刊介绍:
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.