利用高效薄层色谱法开发和验证水溶性维生素定量分析方法及其在营养保健品分析中的应用

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-07-04 DOI:10.3390/separations11070207

K. M. Y. K. Sikdar, Md Khairul Islam, Tomislav Sostaric, L. Y. Lim, C. Locher

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引用次数: 0

摘要

本研究以乙醇-水(70:30, v/v)为流动相，建立了基于高效薄层色谱(HPTLC)的5种水溶性维生素B2、B3、B6、B12和C的定量分析方法。该方法的检出限/定量限分别为每波段5.27/15.97纳克（维生素B2）、13.03/39.47纳克（维生素B3）、59.61/180.64纳克（维生素B6）、14.17/42.93纳克（维生素B12）和119.27/361.42纳克（维生素C）。该方法成功地定量分析了一系列营养保健品中的五种维生素，证明了其在质量控制方面的实用性。

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Development and Validation of a Quantitative Analysis of Water-Soluble Vitamins Using High-Performance Thin-Layer Chromatography and Its Application to the Analysis of Nutraceuticals

In this study, a high-performance thin-layer chromatography (HPTLC)-based method was developed for the quantification of five water-soluble vitamins, B2, B3, B6, B12, and C, using ethanol–water (70:30, v/v) as the mobile phase. The method was validated according to the International Conference on Harmonisation (ICH) guidelines and the limits of detection/limits of quantification were determined as 5.27/15.97 ng (Vitamin B2), 13.03/39.47 ng (Vitamin B3), 59.61/180.64 ng (Vitamin B6), 14.17/42.93 ng (Vitamin B12), and 119.27/361.42 ng (Vitamin C) per band, respectively. The usefulness of the validated method for quality control was demonstrated with the successful quantitative analysis of the five vitamins in a range of nutraceuticals.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.