犀牛叶提取物及化学成分的植物化学、抗酪氨酸酶和抗糖尿病研究

Q3 Chemistry Chemistry Pub Date : 2024-07-02 DOI:10.3390/chemistry6040032
Olusola Ifedolapo Watti, Masande Yalo, Rajan Sharma, Masixole Makhaba, Ahmed A. Hussein, W. Mabusela
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引用次数: 0

摘要

Dicerothamnus rhinocerotis (L.f.) Koekemoer,又名犀牛灌木,以前叫 Elytropappus rhinocerotis (L.f.) Less.,属于菊科植物。该植物传统上用于治疗消化不良、胃溃疡、流感和腹泻。本研究旨在调查 D. rhinocerotis 的植物化学、抗葡萄糖苷酶、抗淀粉酶和抗酪氨酸酶作用,因为这方面的研究还很有限。风干的植物材料在 80% 的甲醇(MeOH)中浸渍,然后在正己烷、二氯甲烷(DCM)、乙酸乙酯(EtOAc)和丁醇(BuOH)中分馏。采用硅胶柱色谱法分离化合物。从馏分中一共分离出六种化合物(1-6),即:醋酸苷(1)、15-羟基-顺式-3-克勒罗丹烯-18-酸(2)、醋酸苷-7-葡萄糖苷(3)、蒎烷醇(4)、芹菜甙(5)和β-谷甾醇-3-O-糖苷(6)。化合物 2-4 和 6 是首次从这种植物中提取。在不同的馏分中,BuOH 和 EtOAc 馏分具有很强的酪氨酸酶抑制活性,IC50 值分别为 13.7 ± 1.71 和 11.6 ± 2.68 µg/mL,而在分离出的化合物中,芹菜素(5)的抑制活性最强,IC50 值为 14.58 µM,与 Kojic 酸(17.26 µM)的抑制活性相当。抗葡萄糖苷酶试验表明,三种馏分和化合物 5 具有良好的活性,而抗淀粉酶试验则未显示出明显的抑制活性。
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Phytochemistry, Anti-Tyrosinase, and Anti-Diabetes Studies of Extracts and Chemical Constituents of Dicerothamnus rhinocerotis Leaves
Dicerothamnus rhinocerotis (L.f.) Koekemoer, also known as rhinoceros bush and previously called Elytropappus rhinocerotis (L.f.) Less., is from the Asteraceae plant family. The plant is traditionally used to treat indigestion, stomach ulcers, influenza, and diarrhea. This study was aimed at investigating the phytochemistry, anti-glucosidase, anti-amylase, and anti-tyrosinase effects of D. rhinocerotis as research in this area is limited. The air-dried plant materials were macerated in 80% methanol (MeOH) and fractionated between hexane, dichloromethane (DCM), ethyl acetate (EtOAc), and butanol (BuOH). Column chromatography on silica gel was employed for the isolation of the compounds. A total of six compounds (1–6) were isolated from the fractions viz. acacetin (1), 15-hydroxy-cis-clerodan-3-ene-18-oic-acid (2), acacetin-7-glucoside (3), pinitol (4), apigenin (5), and β-sitosterol-3-O-glycoside (6). Compounds 2–4 and 6 are reported for the first time from this plant. Among the different fractions, the BuOH and EtOAc fractions had strong tyrosinase inhibitory activities with IC50 values of 13.7 ± 1.71 and 11.6 ± 2.68 µg/mL, respectively, while among the isolated compounds, apigenin (5) had the strongest inhibitory activity, with an IC50 of 14.58 µM, which competes favorably with Kojic acid (17.26 µM). The anti-glucosidase assay showed good activity in three of the fractions and compound 5, while the anti-amylase assays did not show significant inhibition activity.
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来源期刊
CiteScore
2.50
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0.00%
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审稿时长
11 weeks
期刊介绍: Chemistry—A European Journal is a truly international journal with top quality contributions (2017 ISI Impact Factor: 5.16). It publishes a wide range of outstanding Reviews, Minireviews, Concepts, Full Papers, and Communications from all areas of chemistry and related fields.
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