{"title":"Streptococcus suis subsp:从人类临床标本和野猪口腔样本中分离出的兰斯菲尔德A组抗原阳性菌","authors":"Yuki Hasegawa , Toyokazu Akita , Tomokazu Kuchibiro , Tohru Miyoshi-Akiyama , Junko Tomida , Ryo Kutsuna , Ryota Mori , Miki Okuno , Yoshitoshi Ogura , Yoshiaki Kawamura","doi":"10.1016/j.syapm.2024.126538","DOIUrl":null,"url":null,"abstract":"<div><p>Three <em>Streptococcus suis</em>-like strains positive for Lancefield antigen group A were isolated from human boar bite wounds and the oral cavities of boars in Hashimoto City, Wakayama Prefecture, Japan, and their taxonomic positions were investigated. Application of the VITEK2 system identified all three isolates as <em>S. suis</em> with > 94 % probability. The isolates were assigned to <em>S. suis</em> based on the results of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis (Biotyper score of 2.382) but were differentiated according to the characteristic signal peaks (4709 <em>m</em>/<em>z</em> and 9420 <em>m</em>/<em>z</em>) that were not present for <em>S. suis</em>. Sequence analysis of the 16S rRNA and <em>sodA</em> genes determined that the isolates were similar to <em>S. suis</em>; however, these genes appeared on a phylogenetic sub-branch. Phylogenetic analysis of the whole chromosomal DNA showed that the isolate formed a cluster with <em>S. suis</em> but with clear divergence. The average nucleotide index using BLAST between the clinical isolate (PAGU 2482) and a closely related reference strain of <em>S. suis</em> was 94.75 %, which was not clearly conclusive; however, digital DNA-DNA hybridization showed a value of 61.2 %. Biochemical reactions, including those with acid phosphatase, α-chymotrypsin, and tagatose (acidification), distinguished our isolates from <em>S. suis</em>. Thus, based on phylogenetic, genomic, and phenotypic characteristics and MALDI-TOF-MS signal patterns, we propose that the isolate with Lancefield group A positive characteristics be designated as a novel subspecies, <em>Streptococcus suis</em> subsp. <em>hashimotonensis</em> subsp. nov., with the type strain PAGU 2482<sup>T</sup> (GTC 18290<sup>T</sup> = CCUG 77434<sup>T</sup>).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"47 5","pages":"Article 126538"},"PeriodicalIF":3.3000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Streptococcus suis subsp. hashimotonensis subsp. nov.: Lancefield group A antigen–positive organisms isolated from human clinical specimens and wild boar oral cavity samples\",\"authors\":\"Yuki Hasegawa , Toyokazu Akita , Tomokazu Kuchibiro , Tohru Miyoshi-Akiyama , Junko Tomida , Ryo Kutsuna , Ryota Mori , Miki Okuno , Yoshitoshi Ogura , Yoshiaki Kawamura\",\"doi\":\"10.1016/j.syapm.2024.126538\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Three <em>Streptococcus suis</em>-like strains positive for Lancefield antigen group A were isolated from human boar bite wounds and the oral cavities of boars in Hashimoto City, Wakayama Prefecture, Japan, and their taxonomic positions were investigated. Application of the VITEK2 system identified all three isolates as <em>S. suis</em> with > 94 % probability. The isolates were assigned to <em>S. suis</em> based on the results of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis (Biotyper score of 2.382) but were differentiated according to the characteristic signal peaks (4709 <em>m</em>/<em>z</em> and 9420 <em>m</em>/<em>z</em>) that were not present for <em>S. suis</em>. Sequence analysis of the 16S rRNA and <em>sodA</em> genes determined that the isolates were similar to <em>S. suis</em>; however, these genes appeared on a phylogenetic sub-branch. Phylogenetic analysis of the whole chromosomal DNA showed that the isolate formed a cluster with <em>S. suis</em> but with clear divergence. The average nucleotide index using BLAST between the clinical isolate (PAGU 2482) and a closely related reference strain of <em>S. suis</em> was 94.75 %, which was not clearly conclusive; however, digital DNA-DNA hybridization showed a value of 61.2 %. Biochemical reactions, including those with acid phosphatase, α-chymotrypsin, and tagatose (acidification), distinguished our isolates from <em>S. suis</em>. Thus, based on phylogenetic, genomic, and phenotypic characteristics and MALDI-TOF-MS signal patterns, we propose that the isolate with Lancefield group A positive characteristics be designated as a novel subspecies, <em>Streptococcus suis</em> subsp. <em>hashimotonensis</em> subsp. nov., with the type strain PAGU 2482<sup>T</sup> (GTC 18290<sup>T</sup> = CCUG 77434<sup>T</sup>).</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"47 5\",\"pages\":\"Article 126538\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202024000523\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202024000523","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
从日本和歌山县桥本市的人被野猪咬伤的伤口和野猪的口腔中分离出了三株对兰斯菲尔德抗原 A 群呈阳性的猪链球菌样菌株,并对它们的分类位置进行了研究。应用 VITEK2 系统将所有三个分离物鉴定为猪链球菌的概率为 > 94 %。根据基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)分析的结果(Biotyper 得分为 2.382),这些分离物被归类为 S. suis,但根据 S. suis 不存在的特征信号峰(4709 m/z 和 9420 m/z)进行了区分。16S rRNA 和 sodA 基因的序列分析表明,这些分离物与猪痢疾杆菌相似;但是,这些基因出现在一个系统发育子分支上。对整个染色体 DNA 的系统进化分析表明,该分离物与 S. suis 形成了一个群集,但有明显的差异。使用 BLAST 对临床分离株(PAGU 2482)和与之密切相关的猪链球菌参考株之间的核苷酸平均指数进行了分析,结果为 94.75%,这并不能明确得出结论;然而,数字 DNA-DNA 杂交的结果显示该值为 61.2%。生化反应,包括酸性磷酸酶、α-糜蛋白酶和塔格糖(酸化)反应,将我们的分离物与猪链球菌区分开来。因此,根据系统发育、基因组和表型特征以及 MALDI-TOF-MS 信号模式,我们建议将具有 Lancefield A 组阳性特征的分离物定为一个新亚种,即猪链球菌 hashimotonensis 亚种,模式菌株为 PAGU 2482T (GTC 18290T = CCUG 77434T)。
Streptococcus suis subsp. hashimotonensis subsp. nov.: Lancefield group A antigen–positive organisms isolated from human clinical specimens and wild boar oral cavity samples
Three Streptococcus suis-like strains positive for Lancefield antigen group A were isolated from human boar bite wounds and the oral cavities of boars in Hashimoto City, Wakayama Prefecture, Japan, and their taxonomic positions were investigated. Application of the VITEK2 system identified all three isolates as S. suis with > 94 % probability. The isolates were assigned to S. suis based on the results of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis (Biotyper score of 2.382) but were differentiated according to the characteristic signal peaks (4709 m/z and 9420 m/z) that were not present for S. suis. Sequence analysis of the 16S rRNA and sodA genes determined that the isolates were similar to S. suis; however, these genes appeared on a phylogenetic sub-branch. Phylogenetic analysis of the whole chromosomal DNA showed that the isolate formed a cluster with S. suis but with clear divergence. The average nucleotide index using BLAST between the clinical isolate (PAGU 2482) and a closely related reference strain of S. suis was 94.75 %, which was not clearly conclusive; however, digital DNA-DNA hybridization showed a value of 61.2 %. Biochemical reactions, including those with acid phosphatase, α-chymotrypsin, and tagatose (acidification), distinguished our isolates from S. suis. Thus, based on phylogenetic, genomic, and phenotypic characteristics and MALDI-TOF-MS signal patterns, we propose that the isolate with Lancefield group A positive characteristics be designated as a novel subspecies, Streptococcus suis subsp. hashimotonensis subsp. nov., with the type strain PAGU 2482T (GTC 18290T = CCUG 77434T).
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: