{"title":"通过织物检测血液、精液和唾液:试点研究","authors":"","doi":"10.1016/j.forsciint.2024.112153","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to identify if biological material could be detected on the opposite side to deposition on fabric by commonly used presumptive and/or secondary tests. Additionally, this study aimed to ascertain if there is a difference in the DNA quantity and quality from samples obtained from both sides of the same substrate: cotton, polyester, denim, or combined viscose and polyester swatches. Blood, semen, or saliva (25 μL) was deposited on one side of 5 replicates of each fabric type and left for 24 h. Blood swatches were tested using Hemastix® and the ABACard® HemaTrace® immunoassay, semen swatches were tested using acid phosphatase (AP) reagent, the ABACard® p30® immunoassay and hematoxylin and eosin staining, and saliva swatches were tested using Phadebas® paper and the RSID-Saliva™ immunoassay. Both sides of each swatch were separately wet/dry swabbed and subjected to DNA analysis. Blood was able to be detected on the underside of all fabrics using both tests. Semen was able to be detected on the underside of swatches using the presumptive AP test but not p30®, and sperm was rarely observed. Saliva was able to be detected by RSID-Saliva™ but not Phadebas® paper when the underside of swatches were tested. Across all biological materials, DNA was able to be recovered from the top side of all 60 swatches. For the underside, DNA was able to be recovered from 54 swatches. Of the 6 swatches that DNA was unable to be recovered from, one sample was from semen and the rest were from saliva. This study has demonstrated that DNA and components of interest in forensically relevant biological material can be recovered from the opposite side to where it was originally deposited, and that observing biological material and/or DNA on one side of fabric does not definitively indicate direct deposition on that side.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0379073824002342/pdfft?md5=1a380eab9eb0f7a71f5b73eaea81a060&pid=1-s2.0-S0379073824002342-main.pdf","citationCount":"0","resultStr":"{\"title\":\"The detection of blood, semen and saliva through fabrics: A pilot study\",\"authors\":\"\",\"doi\":\"10.1016/j.forsciint.2024.112153\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to identify if biological material could be detected on the opposite side to deposition on fabric by commonly used presumptive and/or secondary tests. Additionally, this study aimed to ascertain if there is a difference in the DNA quantity and quality from samples obtained from both sides of the same substrate: cotton, polyester, denim, or combined viscose and polyester swatches. Blood, semen, or saliva (25 μL) was deposited on one side of 5 replicates of each fabric type and left for 24 h. Blood swatches were tested using Hemastix® and the ABACard® HemaTrace® immunoassay, semen swatches were tested using acid phosphatase (AP) reagent, the ABACard® p30® immunoassay and hematoxylin and eosin staining, and saliva swatches were tested using Phadebas® paper and the RSID-Saliva™ immunoassay. Both sides of each swatch were separately wet/dry swabbed and subjected to DNA analysis. Blood was able to be detected on the underside of all fabrics using both tests. Semen was able to be detected on the underside of swatches using the presumptive AP test but not p30®, and sperm was rarely observed. Saliva was able to be detected by RSID-Saliva™ but not Phadebas® paper when the underside of swatches were tested. Across all biological materials, DNA was able to be recovered from the top side of all 60 swatches. For the underside, DNA was able to be recovered from 54 swatches. Of the 6 swatches that DNA was unable to be recovered from, one sample was from semen and the rest were from saliva. 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引用次数: 0
摘要
本研究旨在通过常用的推定和/或辅助测试,确定能否在织物上沉积物的反面检测到生物材料。此外,本研究还旨在确定从同一基材(棉、涤纶、牛仔布或粘胶和涤纶组合色板)正反两面获得的样本在 DNA 数量和质量上是否存在差异。将血液、精液或唾液(25 μL)涂抹在每种织物的 5 个重复样本的一面,静置 24 小时。血液样本使用 Hemastix® 和 ABACard® HemaTrace® 免疫测定法进行检测;精液样本使用酸性磷酸酶(AP)试剂、ABACard® p30® 免疫测定法和苏木精及伊红染色法进行检测;唾液样本使用 Phadebas® 纸和 RSID-Saliva™ 免疫测定法进行检测。每份唾液样本的两面都分别进行了干/湿拭抹,并进行了 DNA 分析。使用这两种检测方法,所有织物的底面都能检测到血液。使用推定 AP 检验法可在棉样底部检测到精液,但 p30® 检验法检测不到精液,而且很少观察到精子。用 RSID-Saliva™ 检测棉样底面时,能检测到唾液,但不能检测到 Phadebas® 纸。在所有生物材料中,所有 60 块棉样的顶面都能回收 DNA。在底面检测中,有 54 块试样可以提取到 DNA。在无法从 6 个样本中提取 DNA 的样本中,一个样本来自精液,其余来自唾液。这项研究表明,与法医相关的生物材料中的 DNA 和相关成分可以从其最初沉积的反面回收,而且在织物的一面观察到生物材料和/或 DNA 并不能明确表明该面是直接沉积的。
The detection of blood, semen and saliva through fabrics: A pilot study
This study aimed to identify if biological material could be detected on the opposite side to deposition on fabric by commonly used presumptive and/or secondary tests. Additionally, this study aimed to ascertain if there is a difference in the DNA quantity and quality from samples obtained from both sides of the same substrate: cotton, polyester, denim, or combined viscose and polyester swatches. Blood, semen, or saliva (25 μL) was deposited on one side of 5 replicates of each fabric type and left for 24 h. Blood swatches were tested using Hemastix® and the ABACard® HemaTrace® immunoassay, semen swatches were tested using acid phosphatase (AP) reagent, the ABACard® p30® immunoassay and hematoxylin and eosin staining, and saliva swatches were tested using Phadebas® paper and the RSID-Saliva™ immunoassay. Both sides of each swatch were separately wet/dry swabbed and subjected to DNA analysis. Blood was able to be detected on the underside of all fabrics using both tests. Semen was able to be detected on the underside of swatches using the presumptive AP test but not p30®, and sperm was rarely observed. Saliva was able to be detected by RSID-Saliva™ but not Phadebas® paper when the underside of swatches were tested. Across all biological materials, DNA was able to be recovered from the top side of all 60 swatches. For the underside, DNA was able to be recovered from 54 swatches. Of the 6 swatches that DNA was unable to be recovered from, one sample was from semen and the rest were from saliva. This study has demonstrated that DNA and components of interest in forensically relevant biological material can be recovered from the opposite side to where it was originally deposited, and that observing biological material and/or DNA on one side of fabric does not definitively indicate direct deposition on that side.
期刊介绍:
Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law.
The journal publishes:
Case Reports
Commentaries
Letters to the Editor
Original Research Papers (Regular Papers)
Rapid Communications
Review Articles
Technical Notes.