揭示细胞内的相分离:生物分子凝聚物光学成像的进展。

IF 11.6 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Trends in Biochemical Sciences Pub Date : 2024-10-01 DOI:10.1016/j.tibs.2024.06.014
Yinfeng Guo (郭寅风) , Xin Zhang (张鑫)
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引用次数: 0

摘要

通过相分离形成的细胞内生物分子凝聚体显示出高度有序的超微结构和复杂的特性。光学成像技术的最新进展,包括超分辨率显微镜和利用所观察分子固有特性的创新显微方法,已经超越了传统显微镜的局限性。这些进步有助于以更精细的尺度和更详细的细节探索凝聚态。利用这些新兴而复杂的成像工具,可以精确观测这些凝聚体的多相组织和理化性质,从而揭示它们在细胞过程中的功能。在这篇综述中,我们将重点介绍方法创新的最新进展及其对理解细胞内生物分子凝聚体的组织和动力学的深远影响。
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Unveiling intracellular phase separation: advances in optical imaging of biomolecular condensates
Intracellular biomolecular condensates, which form via phase separation, display a highly organized ultrastructure and complex properties. Recent advances in optical imaging techniques, including super-resolution microscopy and innovative microscopic methods that leverage the intrinsic properties of the molecules observed, have transcended the limitations of conventional microscopies. These advances facilitate the exploration of condensates at finer scales and in greater detail. The deployment of these emerging but sophisticated imaging tools allows for precise observations of the multiphasic organization and physicochemical properties of these condensates, shedding light on their functions in cellular processes. In this review, we highlight recent progress in methodological innovations and their profound implications for understanding the organization and dynamics of intracellular biomolecular condensates.
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来源期刊
Trends in Biochemical Sciences
Trends in Biochemical Sciences 生物-生化与分子生物学
CiteScore
22.90
自引率
0.70%
发文量
148
审稿时长
6-12 weeks
期刊介绍: For over 40 years, Trends in Biochemical Sciences (TIBS) has been a leading publication keeping readers informed about recent advances in all areas of biochemistry and molecular biology. Through monthly, peer-reviewed issues, TIBS covers a wide range of topics, from traditional subjects like protein structure and function to emerging areas in signaling and metabolism. Articles are curated by the Editor and authored by top researchers in their fields, with a focus on moving beyond simple literature summaries to providing novel insights and perspectives. Each issue primarily features concise and timely Reviews and Opinions, supplemented by shorter articles including Spotlights, Forums, and Technology of the Month, as well as impactful pieces like Science & Society and Scientific Life articles.
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