通过组织工程学开发一种可生物降解的假体,用于器官替代或肝外胆管替代。

IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Annals of hepatology Pub Date : 2024-07-20 DOI:10.1016/j.aohep.2024.101530
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引用次数: 0

摘要

导言和目的:在不同的情况下,需要进行肝外胆管置换或替代,如胆管癌初期和局部阶段、胆管缺失、胆管狭窄或胆管中断。材料和方法:开发了一种由聚(D,L-内酯-共聚乙二醇)(PGLA)-聚己内酯(PCL)-明胶(Gel)组成的电纺丝假体,并进行了机械和生物测试,以评估其抗张力、生物相容性、生物降解性、细胞毒性、形态分析和细胞培养。将获得的假体植入 15 头猪的肝外胆管,并进行了为期两年的随访。结果:机械和生物评估表明,这种支架具有生物相容性和生物降解性。在实验模型中植入的假体允许细胞粘附、迁移和增殖,在不改变肝功能检测结果的情况下保持了胆管的通透性。免疫组化分析表明存在胆道上皮。结论:由电纺PGLA-PCL-Gel纳米纤维组成的管状支架首次用于替代猪的肝外胆管。机械和生物学评估表明,这种支架具有生物相容性和生物可降解性,是用于胆管和其他潜在组织工程应用的绝佳候选材料。
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Development of a biodegradable prosthesis through tissue engineering, for the organ-replacement or substitution of the extrahepatic bile duct

Introduction and Objectives

There are different situations in which an extrahepatic bile duct replacement or substitute is needed, such as initial and localized stages of bile duct cancer, agenesis, stenosis, or bile duct disruption.

Materials and Methods

A prosthesis obtained by electrospinning composed of Poly (D,L-lactide-co-glycolide) (PGLA) - Polycaprolactone (PCL) - Gelatin (Gel) was developed, mechanical and biological tests were carried out to evaluate resistance to tension, biocompatibility, biodegradability, cytotoxicity, morphological analysis and cell culture. The obtained prosthesis was placed in the extrahepatic bile duct of 15 pigs with a 2-year follow-up. Liver function tests and cholangioscopy were evaluated during follow-up.

Results

Mechanical and biological evaluations indicate that this scaffold is biocompatible and biodegradable. The prosthesis implanted in the experimental model allowed cell adhesion, migration, and proliferation, maintaining bile duct permeability without altering liver function tests. Immunohistochemical analysis indicates the presence of biliary epithelium.

Conclusions

A tubular scaffold composed of electrospun PGLA-PCL-Gel nanofibers was used for the first time to replace the extrahepatic bile duct in pigs. Mechanical and biological evaluations indicate that this scaffold is biocompatible and biodegradable, making it an excellent candidate for use in bile ducts and potentially in other tissue engineering applications.

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来源期刊
Annals of hepatology
Annals of hepatology 医学-胃肠肝病学
CiteScore
7.90
自引率
2.60%
发文量
183
审稿时长
4-8 weeks
期刊介绍: Annals of Hepatology publishes original research on the biology and diseases of the liver in both humans and experimental models. Contributions may be submitted as regular articles. The journal also publishes concise reviews of both basic and clinical topics.
期刊最新文献
Editorial board Global multi-societies endorsement of the MAFLD definition An Acknowledgement Biological aging accelerates hepatic fibrosis: Insights from the NHANES 2017-2020 and genome-wide association study analysis. Development of a biodegradable prosthesis through tissue engineering, for the organ-replacement or substitution of the extrahepatic bile duct
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