优化基于肽折叠聚合物的人工逆醛酸酶

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL ACS Applied Energy Materials Pub Date : 2024-08-12 DOI:10.1039/d4cy00342j

Katarzyna Ożga , Ewa Rudzińska-Szostak , Łukasz Berlicki

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引用次数: 0

摘要

肽折叠体具有可预测和可控制的三维结构，是一类有利于开发功能分子的化合物。其中最具挑战性的应用之一是构建类似酶的催化剂。在这里，我们介绍了如何优化由两个 9/12/9/10 螺旋组成的多肽折叠体，使其具有顺式-2-氨基环戊烷羧酸残基的逆醛醇活性。通过对螺旋手性、螺旋间连接刚性和活性位点结构的修改，产生了高活性的逆醛酸酶模拟物。核磁共振测量证实了活性位点残基的假定排列。

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Optimization of peptide foldamer-based artificial retro-aldolase†

Due to their predictable and controllable three-dimensional structure, peptide foldamers constitute a class of compounds beneficial for developing functional molecules. One of the most challenging applications is the construction of enzyme-like catalysts. Here, we describe the optimization of peptide foldamers composed of two 9/12/9/10-helices incorporating cis-2-aminocyclopentanecarboxylic acid residues toward retro-aldol activity. Modifications related to helix handedness, interhelical linker rigidity, and active site construction led to highly active retro-aldolase mimetics. NMR measurements confirmed the assumed arrangement of active site residues.

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.