矮牵牛素通过调节Th1/Th17平衡和氧化应激抑制桥本氏甲状腺炎

IF 3.7 4区 医学 Q2 CELL BIOLOGY Cellular immunology Pub Date : 2024-07-17 DOI:10.1016/j.cellimm.2024.104858
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引用次数: 0

摘要

桥本氏甲状腺炎(HT)是一种常见的自身免疫性甲状腺疾病,需要进一步研究以确定有效的治疗策略。炎症和氧化应激是桥本氏甲状腺炎的两个关键病理生理因素。矮牵牛素(PET)是一种具有抗炎和抗氧化特性的花青素。本研究旨在探讨 PET 对 HT 的影响和机制。用甲状腺球蛋白乳化佐剂注射 C57BL/6N 小鼠,建立 HT 动物模型。结果表明,PET能降低HT小鼠体内TPOAb、TgAb、T3、T4、IgG、IgA和IgM的浓度,同时还能显著改变滤泡形态,增加淋巴细胞浸润。此外,HT小鼠的细胞凋亡率、ROS水平、MDA含量、CD4+水平、IFN-γ和IL-17A水平以及IFN-γ和IL-17的浓度均升高,PET治疗可降低其浓度。此外,HT 患者的 NOX4 和 PKM2 水平较高,与 TPOAb、IFN-γ 和 IL-17 的浓度呈正相关。在 HT 小鼠中,PET 治疗降低了 PKM2 和 NOX4 蛋白的表达。总之,PET可通过调节NOX4/PKM2轴抑制HT小鼠的细胞凋亡、氧化应激和Th1/Th17分化,从而改善甲状腺功能障碍,这表明PET在HT干预中具有广阔的应用前景。
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Petunidin suppresses Hashimoto’s thyroiditis by regulating Th1/Th17 homeostasis and oxidative stress

Hashimoto’s thyroiditis (HT) is a prevalent autoimmune thyroid disease, necessitating further research to identify effective treatment strategies. Two key pathophysiological factors of HT are inflammation and oxidative stress. Petunidin (PET) is an anthocyanin with anti-inflammatory and antioxidant properties. This study aimed to investigate the effect and mechanism of PET on HT. C57BL/6N mice were injected with thyroglobulin emulsified with adjuvant to establish the HT animal model. Our results showed that PET administration decreased the concentrations of TPOAb, TgAb, T3, T4, IgG, IgA and IgM in HT mice, accompanied by significant alterations in follicle shape and increased lymphocyte infiltrations. Additionally, the apoptosis rate, ROS level, MDA content, CD4+ level, IFN-γ and IL-17A levels, as well as the concentrations of IFN-γ and IL-17, were elevated in HT mice and reduced by PET treatment. Furthermore, HT patients exhibited higher levels of NOX4 and PKM2, which were positively correlated with TPOAb, IFN-γ, and IL-17 concentrations. In HT mice, PET therapy decreased the expression of PKM2 and NOX4 proteins. In summary, PET can improve thyroid dysfunction by suppressing apoptosis, oxidative stress and Th1/Th17 differentiation through regulation of the NOX4/PKM2 axis in HT mice, suggesting its promising potential for HT intervention.

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来源期刊
Cellular immunology
Cellular immunology 生物-免疫学
CiteScore
8.20
自引率
2.30%
发文量
102
审稿时长
30 days
期刊介绍: Cellular Immunology publishes original investigations concerned with the immunological activities of cells in experimental or clinical situations. The scope of the journal encompasses the broad area of in vitro and in vivo studies of cellular immune responses. Purely clinical descriptive studies are not considered. Research Areas include: • Antigen receptor sites • Autoimmunity • Delayed-type hypersensitivity or cellular immunity • Immunologic deficiency states and their reconstitution • Immunologic surveillance and tumor immunity • Immunomodulation • Immunotherapy • Lymphokines and cytokines • Nonantibody immunity • Parasite immunology • Resistance to intracellular microbial and viral infection • Thymus and lymphocyte immunobiology • Transplantation immunology • Tumor immunity.
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