外细胞生物水解酶 CBHA 对烟叶发酵的影响

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-19 DOI:10.4014/jmb.2404.04028
Xueqin Xu, Qianqian Wang, Longyan Yang, Zhiyan Chen, Yun Zhou, Hui Feng, Peng Zhang, Jie Wang
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引用次数: 0

摘要

烟草质量直接受大分子含量的影响,发酵是改善生化特性的有效方法。本研究利用 Pichia pastoris 表达的 CBHA(cellobiohydrolase A)糖化酶作为发酵添加剂。测定了发酵后烟叶中主要化学成分的含量,分析了发酵过程中烟叶中微生物群落结构和丰度的变化。研究了化学成分与微生物组成变化之间的关系,并预测了细菌和真菌在发酵过程中的功能,确定了可能的代谢途径。CBHA发酵48小时后,烟叶中淀粉、纤维素和总氮的含量分别下降了17.60%、28.91%和16.05%。微生物群落结构发生了显著变化,CBHA 处理组曲霉菌数量明显减少,而 Filobasidum、Cladosporium、Bullera、Komagataella 等有所增加。可溶性糖受烟叶微生物群落的影响最大,与淀粉、纤维素和总氮呈负相关。在发酵过程中,代谢相关功能基因的相对丰度增加,纤维素酶和内肽酶的表达量也有所增加。结果表明,烟叶上细菌群落和优势微生物群落的变化影响了烟叶中化学成分的含量,添加 CBHA 进行发酵对提高烟叶质量有积极作用。
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Effects of Exocellobiohydrolase CBHA on Fermentation of Tobacco Leaves.

The quality of tobacco is directly affected by macromolecular content, fermentation is an effective method to improve biochemical properties. In this study, we utilized CBHA (cellobiohydrolase A) glycosylase, which was expressed by Pichia pastoris, as an additive for fermentation. The contents of main chemical components of tobacco leaves after fermentation were determined, and the changes of microbial community structure and abundance in tobacco leaves during fermentation were analyzed. The relationship between chemical composition and changes in microbial composition was investigated, and the function of bacteria and fungi in fermentation was predicted to identify possible metabolic pathways. After 48 h of CBHA fermentation, the contents of starch, cellulose and total nitrogen in tobacco leaf decreased by 17.60%, 28.91% and 16.05%, respectively. The microbial community structure changed significantly, with Aspergillus abundance decreasing significantly, while Filobasidum, Cladosporium, Bullera, Komagataella, etc., increased in CBHA treated group. Soluble sugar was most affected by microbial community in tobacco leaves, which was negatively correlated with starch, cellulose and total nitrogen. During the fermentation process, the relative abundance of metabolism-related functional genes increased, and the expressions of cellulase and endopeptidase also increased. The results showed that the changes of bacterial community and dominant microbial community on tobacco leaves affected the content of chemical components in tobacco leaves, and adding CBHA for fermentation had a positive effect on improving the quality of tobacco leaves.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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