评估作为潜在根管灌洗剂的精油的抗菌和抗生物膜特性的体外方法--技术步骤简述。

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS Methods and Protocols Pub Date : 2024-06-25 DOI:10.3390/mps7040050
Jihad Diouchi, Jelena Marinković, Milica Nemoda, Lhoussaine El Rhaffari, Babacar Toure, Sonia Ghoul
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引用次数: 0

摘要

背景:精油因其多种生物活性而变得越来越重要,许多行业对精油的需求也在不断增长。本文重点介绍了对精油的抗菌和抗生物膜活性进行体外评估的技术步骤,以便将其用作牙科根管冲洗剂:方法:通过体外试验研究了精油的生物活性。以革兰氏阳性菌粪肠球菌为微生物模型。精油的抗菌活性采用微量稀释法和树脂醛染色法进行评估,以确定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。采用微孔板培养技术和水晶紫染色法,在 570 纳米波长处用分光光度法评估了抗生物膜效果:本文提供了一个详细的体外实验方案,便于制备精油样品、细菌悬浮液以及用于评估精油抗生物膜和抗菌活性的方法。文章介绍了这些方法的优点,以及与细菌和精油的选择有关的限制。
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In Vitro Methods for Assessing the Antibacterial and Antibiofilm Properties of Essential Oils as Potential Root Canal Irrigants-A Simplified Description of the Technical Steps.

Background: Essential oils have gained in significance due to their various biological activities, and there is a growing demand for them in many industries. The present article focuses on the technical steps for an in vitro evaluation of the antibacterial and antibiofilm activities of essential oils for potential use as root canal irrigant in dentistry.

Methods: The bioactivities of the essential oil were investigated through in vitro assays. The gram-positive bacterium Enterococcus faecalis was used as a micro-organism model. The antibacterial activity of the essential oil was assessed using the microdilution method, and resazurin staining to determine the minimal inhibition concentrations (MICs) and the minimal bactericidal concentrations (MBCs). The antibiofilm effect was evaluated spectrophotometrically at 570 nm using the microplate cultivation technique and crystal violet staining.

Conclusions: This article features a detailed in vitro protocol to facilitate the preparation of the essential oil samples, the bacterial suspension, and the methods used for assessment of the antibiofilm and antibacterial activities of the essential oil. The advantages of these approaches are presented in relation to the limits linked to the choice of the bacteria and the essential oil.

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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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