注射卫星肌母细胞和间充质干细胞可减少大鼠肩袖撕裂后的脂肪变性。

IF 2 Q2 ORTHOPEDICS Journal of Experimental Orthopaedics Pub Date : 2024-07-24 DOI:10.1002/jeo2.12087

Tahir Koray Yozgatli, Elif Gelenli Dolanbay, Tunca Cingoz, Ahmet Emre Paksoy, Unal Uslu, Ercument Ovali, Baris Kocaoglu

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引用次数: 0

摘要

目的：肩袖（RC）撕裂会导致脂肪变性，延误治疗会加重病情。单靠手术修复无法逆转脂肪变性。本研究旨在测试在脂肪变性 RC 撕裂的大鼠模型中，局部注射三角肌区域的卫星细胞衍生肌母细胞或卫星肌母细胞（SM）和腹部皮下脂肪垫的间充质干细胞（MSCs）是否会刺激肌生成和减少脂肪生成：方法：在t = 0时对24只Wistar白化大鼠的双肩进行标准化RC撕裂手术，并对大鼠进行为期8周的随访，以建立慢性退变模型。动物被随机分为修复 + SM 和间充质干细胞组（n = 12）或仅修复组（n = 12）。在第8周，对所有大鼠的右肩进行带或不带干细胞注射的经骨修复，并在第9周和第10周进行额外注射。左肩作为对照组。动物随访至第 14 周，以观察恢复情况：结果：第14周进行了组织学和组织形态学分析。修复 + SM 和间充质干细胞组的冈上肌质量明显高于仅修复组和对照组。修复+SM和间充质干细胞组的脂肪组织比率明显低于单纯修复组和对照组：从组织学角度看，修复 + SM 和间充质干细胞组的肌肉和肌腱组织得到了改善。结论：从组织学角度看，修复+SM和间充质干细胞组改善了肌肉和肌腱组织。在治疗大鼠模型中的慢性退行性RC撕裂时，手术修复结合注射SM和间充质干细胞改善了脂肪变性、肌腱愈合和肌生成：证据等级：三级。

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Satellite myoblast and mesenchymal stem cell injections decrease fatty degeneration after rotator cuff tear in rats

Purpose

Rotator cuff (RC) tears cause fatty degeneration, aggravated by delayed treatment. Surgical repair alone cannot reverse fatty degeneration. It was aimed to test if local injections of satellite cell-derived myoblasts or satellite myoblasts (SM) from the deltoid region and mesenchymal stem cells (MSCs) from the subcutaneous abdominal fat pad would stimulate myogenesis and decrease adipogenesis in the rat model of fatty degenerated RC tear.

Methods

A standardized RC tear surgery was performed on both shoulders of 24 Wistar albino rats at t = 0, and rats were followed for 8 weeks to create a chronic degeneration model. The animals were randomly divided into repair + SM and MSC (n = 12) or repair only (n = 12) groups. Transosseous repair with or without stem cell-based injection was performed on the right shoulder of all rats on week 8, with additional injections on weeks 9 and 10. The left shoulders were used as control. The animals were followed until week 14 for recovery.

Results

Histological and histomorphometric analyses were performed in week 14. The repair + SM and MSC group had a significantly greater supraspinatus muscle mass than the repair only and control groups. The adipose tissue ratio was significantly lower in the repair + SM and MSC groups versus the repair only and control groups.

Conclusion

Histologically, the repair + SM and MSC group had improved muscle and tendon organization. In treating chronically degenerated RC tear in a rat model, surgical repair combined with injections of SM and MSC improved fatty degeneration, tendon healing and myogenesis.