美洲长春花提取物通过下调 LINC01133/SLAMF9 促进感染性糖尿病溃疡伤口愈合

IF 4 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE Chinese Journal of Natural Medicines Pub Date : 2024-07-01 DOI:10.1016/S1875-5364(24)60569-8
YANG Yuhang , HUANG Jun , LI Xintian , LIN Renjing , WANG Xiaoyan , XIAO Ge , ZENG Juanni , WANG Zhenquan
{"title":"美洲长春花提取物通过下调 LINC01133/SLAMF9 促进感染性糖尿病溃疡伤口愈合","authors":"YANG Yuhang ,&nbsp;HUANG Jun ,&nbsp;LI Xintian ,&nbsp;LIN Renjing ,&nbsp;WANG Xiaoyan ,&nbsp;XIAO Ge ,&nbsp;ZENG Juanni ,&nbsp;WANG Zhenquan","doi":"10.1016/S1875-5364(24)60569-8","DOIUrl":null,"url":null,"abstract":"<div><p>Wound healing in diabetic ulcers remains a significant clinical challenge, primarily due to bacterial infection and impaired angiogenesis. <em>Periplaneta americana</em> extract (PAE) has been widely used to treat diabetic wounds, yet its underlying mechanisms are not fully understood. This study aimed to elucidate these mechanisms by analyzing long non-coding RNA (lncRNA) expressions in the wound tissues from diabetic anal fistula patients treated with or without PAE, using high-throughput sequencing. Peripheral blood monocytes from patients were differentiated into M0 macrophages with human macrophage colony-stimulating factor (hM-CSF) and subsequently polarized into M1 macrophages with lipopolysaccharide. The results indicated that <em>LINC01133</em> and <em>SLAMF9</em> were downregulated in wound tissues of patients treated with PAE. Furthermore, PAE suppressed M1 macrophage polarization and enhanced human umbilical vein endothelial cell (HUVEC) proliferation, migration, and angiogenesis. These effects were diminished when <em>LINC01133</em> or <em>SLAMF9</em> were overexpressed. Mechanistically, <em>LINC01133</em> was shown to upregulate <em>SLAMF9</em> through interaction with <em>ELAVL1</em>. Overexpression of <em>SLAMF9</em> reversed the effects of <em>LINC01133</em> silencing on macrophage polarization and HUVEC functions. In conclusion, PAE facilitates the healing of infected diabetic ulcers by downregulating the <em>LINC01133</em>/<em>SLAMF9</em> pathway.</p></div>","PeriodicalId":10002,"journal":{"name":"Chinese Journal of Natural Medicines","volume":"22 7","pages":"Pages 608-618"},"PeriodicalIF":4.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Periplaneta americana extract promotes infectious diabetic ulcers wound healing by downregulation of LINC01133/SLAMF9\",\"authors\":\"YANG Yuhang ,&nbsp;HUANG Jun ,&nbsp;LI Xintian ,&nbsp;LIN Renjing ,&nbsp;WANG Xiaoyan ,&nbsp;XIAO Ge ,&nbsp;ZENG Juanni ,&nbsp;WANG Zhenquan\",\"doi\":\"10.1016/S1875-5364(24)60569-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Wound healing in diabetic ulcers remains a significant clinical challenge, primarily due to bacterial infection and impaired angiogenesis. <em>Periplaneta americana</em> extract (PAE) has been widely used to treat diabetic wounds, yet its underlying mechanisms are not fully understood. This study aimed to elucidate these mechanisms by analyzing long non-coding RNA (lncRNA) expressions in the wound tissues from diabetic anal fistula patients treated with or without PAE, using high-throughput sequencing. Peripheral blood monocytes from patients were differentiated into M0 macrophages with human macrophage colony-stimulating factor (hM-CSF) and subsequently polarized into M1 macrophages with lipopolysaccharide. The results indicated that <em>LINC01133</em> and <em>SLAMF9</em> were downregulated in wound tissues of patients treated with PAE. Furthermore, PAE suppressed M1 macrophage polarization and enhanced human umbilical vein endothelial cell (HUVEC) proliferation, migration, and angiogenesis. These effects were diminished when <em>LINC01133</em> or <em>SLAMF9</em> were overexpressed. Mechanistically, <em>LINC01133</em> was shown to upregulate <em>SLAMF9</em> through interaction with <em>ELAVL1</em>. Overexpression of <em>SLAMF9</em> reversed the effects of <em>LINC01133</em> silencing on macrophage polarization and HUVEC functions. In conclusion, PAE facilitates the healing of infected diabetic ulcers by downregulating the <em>LINC01133</em>/<em>SLAMF9</em> pathway.</p></div>\",\"PeriodicalId\":10002,\"journal\":{\"name\":\"Chinese Journal of Natural Medicines\",\"volume\":\"22 7\",\"pages\":\"Pages 608-618\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Natural Medicines\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875536424605698\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Natural Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875536424605698","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0

摘要

糖尿病溃疡的伤口愈合仍然是一项重大的临床挑战,主要原因是细菌感染和血管生成受损。美洲长春花提取物(PAE)已被广泛用于治疗糖尿病伤口,但其潜在机制尚未完全明了。本研究旨在利用高通量测序技术分析糖尿病肛瘘患者伤口组织中长非编码RNA(lncRNA)的表达,从而阐明这些机制。用人巨噬细胞集落刺激因子(hM-CSF)将患者外周血单核细胞分化为M0巨噬细胞,然后用脂多糖将其极化为M1巨噬细胞。结果表明,在接受 PAE 治疗的患者伤口组织中,LINC01133 和 SLAMF9 被下调。此外,PAE抑制了M1巨噬细胞的极化,并增强了人脐静脉内皮细胞(HUVEC)的增殖、迁移和血管生成。当过量表达 LINC01133 或 SLAMF9 时,这些作用会减弱。从机理上讲,LINC01133 是通过与 ELAVL1 相互作用来上调 SLAMF9 的。过表达 SLAMF9 逆转了 LINC01133 沉默对巨噬细胞极化和 HUVEC 功能的影响。总之,PAE 可通过下调 LINC01133/SLAMF9 通路促进感染性糖尿病溃疡的愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Periplaneta americana extract promotes infectious diabetic ulcers wound healing by downregulation of LINC01133/SLAMF9

Wound healing in diabetic ulcers remains a significant clinical challenge, primarily due to bacterial infection and impaired angiogenesis. Periplaneta americana extract (PAE) has been widely used to treat diabetic wounds, yet its underlying mechanisms are not fully understood. This study aimed to elucidate these mechanisms by analyzing long non-coding RNA (lncRNA) expressions in the wound tissues from diabetic anal fistula patients treated with or without PAE, using high-throughput sequencing. Peripheral blood monocytes from patients were differentiated into M0 macrophages with human macrophage colony-stimulating factor (hM-CSF) and subsequently polarized into M1 macrophages with lipopolysaccharide. The results indicated that LINC01133 and SLAMF9 were downregulated in wound tissues of patients treated with PAE. Furthermore, PAE suppressed M1 macrophage polarization and enhanced human umbilical vein endothelial cell (HUVEC) proliferation, migration, and angiogenesis. These effects were diminished when LINC01133 or SLAMF9 were overexpressed. Mechanistically, LINC01133 was shown to upregulate SLAMF9 through interaction with ELAVL1. Overexpression of SLAMF9 reversed the effects of LINC01133 silencing on macrophage polarization and HUVEC functions. In conclusion, PAE facilitates the healing of infected diabetic ulcers by downregulating the LINC01133/SLAMF9 pathway.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Chinese Journal of Natural Medicines
Chinese Journal of Natural Medicines INTEGRATIVE & COMPLEMENTARY MEDICINE-PHARMACOLOGY & PHARMACY
CiteScore
7.50
自引率
4.30%
发文量
2235
期刊介绍: The Chinese Journal of Natural Medicines (CJNM), founded and sponsored in May 2003 by China Pharmaceutical University and the Chinese Pharmaceutical Association, is devoted to communication among pharmaceutical and medical scientists interested in the advancement of Traditional Chinese Medicines (TCM). CJNM publishes articles relating to a broad spectrum of bioactive natural products, leading compounds and medicines derived from Traditional Chinese Medicines (TCM). Topics covered by the journal are: Resources of Traditional Chinese Medicines; Interaction and complexity of prescription; Natural Products Chemistry (including structure modification, semi-and total synthesis, bio-transformation); Pharmacology of natural products and prescription (including pharmacokinetics and toxicology); Pharmaceutics and Analytical Methods of natural products.
期刊最新文献
NO inhibitory constituents from Glycosmis craibii var. glabra Multioxidized polyketides from an endophytic Penicillium sp. YUD17006 associated with Gastrodia elata Dual-function natural products: Farnesoid X receptor agonist/inflammation inhibitor for metabolic dysfunction-associated steatotic liver disease therapy Loganin inhibits the ROS-NLRP3-IL-1β axis by activating the NRF2/HO-1 pathway against osteoarthritis Luteolin ameliorates ulcerative colitis in mice via reducing the depletion of NCR+ILC3 through Notch signaling pathway
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1