设计和评估用于印度鳞状皮肤病监测的双抗原间接酶联免疫吸附试验

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS Journal of virological methods Pub Date : 2024-07-24 DOI:10.1016/j.jviromet.2024.114998
Nabaneeta Smaraki , Sanchay Kumar Biswas , Sonalika Mahajan , Vivek Gairola , Sabahat Gulzar , Poloju Deepa , Kirtika Sharma , Harsh Rajeshbhai Jogi , Sushmita Nautiyal , Ragini Mishra , Sukdeb Nandi , Ravikant Agrawal , K. Mahendran , Karam Pal Singh , Gaurav Kumar Sharma
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引用次数: 0

摘要

瘤状皮肤病(LSD)由瘤状皮肤病病毒(Capripoxvirus)引起,正在亚洲大多数国家迅速蔓延。最近,LSD 与极高的发病率和死亡率有关。直到 2019 年,印度仍未发现 LSD 的踪迹,导致这个拥有如此众多牲畜的国家缺乏本地开发的诊断试剂盒、生物制剂和管理该疾病所需的其他工具。因此,本研究旨在设计并验证一种本土的、具有成本效益的内部 ELISA 方法,用于大规模筛查牛样本中的 LSDV 抗体。在原核系统中表达了编码病毒核心蛋白的病毒主开放阅读框 ORF 095 和 ORF 103,并使用重组抗原鸡尾酒对间接 ELISA(iELISA)进行了优化和验证。在阳性率临界值(PP≥50%)下,计算得出的 iELISA 的相对诊断灵敏度和诊断特异性分别为 96.6% 和 95.1%。发现内部设计的双抗原 iELISA 能有效调查印度不同地理区域的 LSDV 血清流行率。
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Design and assessment of a double antigen indirect ELISA for lumpy skin disease surveillance in India

Lumpy skin disease (LSD), caused by the lumpy skin disease virus of the genus Capripoxvirus, is rapidly emerging across most countries in Asia. Recently, LSD has been linked to very high morbidity and mortality rates. Until 2019, India remained free of LSD, resulting in a lack of locally developed diagnostic kits, biologicals, and other tools necessary for managing the disease in a country with such a large livestock population. Therefore, this study aimed to design and validate an indigenous and cost-effective in-house ELISA for large-scale screening of cattle samples for antibodies to LSDV. The viral major open reading frames ORF 095 and ORF 103 encoding virion core proteins were expressed in a prokaryotic system and the recombinant antigen cocktail was used for optimization and validation of an indirect ELISA (iELISA). The calculated relative diagnostic sensitivity and diagnostic specificity of the iELISA were 96.6 % and 95.1 %, respectively at the cut-off percent positivity (PP≥50 %). The in-house designed double-antigen iELISA was found effective to investigate the seroprevalence of LSDV in various geographical regions of India.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
209
审稿时长
41 days
期刊介绍: The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.
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