利用微流体装置制备姜黄素和槲皮素共包囊脂质体的工艺优化

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2024-07-26 DOI:10.1007/s10404-024-02753-9
Vandana Krishna, Harshita Chitturi, Venkata Vamsi Krishna Venuganti
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引用次数: 0

摘要

本研究旨在使用液滴式微流体装置制备、表征和评估姜黄素和槲皮素共包囊脂质体。使用液滴式微流体装置,在 100 至 160 µl/min 的流速下,按照水相与有机相 9:1、6:1、3:1 和 1:1 的不同流速比,合成了由磷脂酰胆碱和胆固醇制成的姜黄素和槲皮素共包囊脂质体。动态光散射技术表明,在 140 微升/分钟和 160 微升/分钟的流速下,9:1 和 6:1 的流速比分别可提供 200-250 纳米的理想粒度范围和 0.17-0.23 的多分散指数。在流速为 6:1 的条件下,姜黄素和槲皮素的最大封装和负载效率分别为 68 ± 9.2% 和 14 ± 1.8%,以及 36 ± 2.7% 和 7.2 ± 0.5%。利用激光共聚焦扫描显微镜对人口腔癌细胞 FaDu 进行的细胞摄取研究表明,脂质体在 2 小时内被细胞摄取。用含有姜黄素(80 µM)、槲皮素(86 µM)和组合(姜黄素 32 µM 和槲皮素 26 µM)的脂质体孵育 FaDu 细胞后,其存活率分别降至 49 ± 2.2%、69 ± 1.5% 和 47 ± 3.5%。细胞凋亡试验表明,组合脂质体通过诱导细胞凋亡抑制了 FaDu 细胞的生长。总之,基于微流控技术的方法可以制备共包囊脂质体,姜黄素和槲皮素组合脂质体对口腔癌有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Process optimization for preparation of curcumin and quercetin co-encapsulated liposomes using microfluidic device

The aim of this study was to prepare, characterize and evaluate liposomes co-encapsulated with curcumin and quercetin using a droplet-based microfluidic device. Curcumin and quercetin co-encapsulated liposomes made of phosphatidylcholine and cholesterol were synthesized using a droplet-based microfluidic device with different flow rate ratios of 9:1, 6:1, 3:1 and 1:1 of the aqueous to organic phase at 100 to 160 µl/min flow rate. The dynamic light scattering technique showed that 9:1 and 6:1 flow rate ratios at 140 and 160 µl/min flow rates, respectively provide desired particle size range of 200–250 nm and 0.17–0.23 polydispersity index. The greatest encapsulation and loading efficiency achieved for curcumin and quercetin was 68 ± 9.2%, 14 ± 1.8%, and 36 ± 2.7%, 7.2 ± 0.5%, respectively with 6:1 flow rate ratio. Cell uptake studies performed on human oral carcinoma cells, FaDu using confocal laser scanning microscopy showed that the liposomes were taken up within 2 h. Clathrin and caveolin-mediated pathways contribute to the cell uptake of liposomes. The FaDu cell viability was reduced to 49 ± 2.2, 69 ± 1.5 and 47 ± 3.5% after incubation with liposomes containing curcumin (80 µM), quercetin (86 µM) and combination (32 µM of curcumin and 26 µM of quercetin), respectively. Apoptosis assay showed that the combination liposomes inhibit FaDu cell growth through apoptosis induced cell death. In conclusion, co-encapsulated liposomes can be prepared by microfluidics-based method and curcumin and quercetin combination liposomes are effective against oral carcinoma.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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