利用阳离子共轭聚合物的 FRET 光传感器实现地塞米松的无标记和超灵敏检测

Biosensors Pub Date : 2024-07-26 DOI:10.3390/bios14080364
Yizhang Xue, Hangbing Liu, Ye Zhang, Weijun Yang, Huixin Li, Yuxuan Gong, Yubai Zhang, Bo Li, Chang Liu, Yi Li
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引用次数: 0

摘要

地塞米松(Dex)是一种在医疗实践中广泛使用的糖皮质激素,应用范围从过敏和炎症到脑水肿和休克。尽管地塞米松具有治疗作用,但由于其潜在的提高成绩效果,被列为运动员禁用物质。因此,我们亟需一个方便快捷的检测平台,对这种药物进行及时准确的检测。在这项研究中,我们提出了一种利用共轭聚合物(CPs)、阳离子共轭聚合物(CCPs)和基因发现探针(GFs)检测 Dex 的无标记佛斯特共振能量转移(FRET)灵敏传感器平台。该系统利用带正电荷的 CCP 与带负电荷的 DNA 之间的静电相互作用,促进了灵敏而特异的 Dex 检测。无标记 FRET 万向传感器平台在检测 Dex 方面表现出了强大的性能,具有很高的选择性和灵敏度。该系统能有效区分 Dex 和干扰分子,并能在常用运动饮料基质的一定浓度范围内实现稳定检测。总之,无标记 FRET Dex 检测系统为检测不同样品基质中的 Dex 提供了一种简单、经济、高灵敏度的方法。它的简便性和有效性使其成为反兴奋剂工作和其他需要快速、准确检测 Dex 的应用的理想工具。
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Label-Free and Ultra-Sensitive Detection of Dexamethasone Using a FRET Aptasensor Utilizing Cationic Conjugated Polymers
Dexamethasone (Dex) is a widely used glucocorticoid in medical practice, with applications ranging from allergies and inflammation to cerebral edema and shock. Despite its therapeutic benefits, Dex is classified as a prohibited substance for athletes due to its potential performance-enhancing effects. Consequently, there is a critical need for a convenient and rapid detection platform to enable prompt and accurate testing of this drug. In this study, we propose a label-free Förster Resonance Energy Transfer (FRET) aptasensor platform for Dex detection utilizing conjugated polymers (CPs), cationic conjugated polymers (CCPs), and gene finder probes (GFs). The system operates by exploiting the electrostatic interactions between positively charged CCPs and negatively charged DNA, facilitating sensitive and specific Dex detection. The label-free FRET aptasensor platform demonstrated robust performance in detecting Dex, exhibiting high selectivity and sensitivity. The system effectively distinguished Dex from interfering molecules and achieved stable detection across a range of concentrations in a commonly used sports drink matrix. Overall, the label-free FRET Dex detection system offers a simple, cost-effective, and highly sensitive approach for detecting Dex in diverse sample matrices. Its simplicity and effectiveness make it a promising tool for anti-doping efforts and other applications requiring rapid and accurate Dex detection.
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