用于比色铜传感、溶酶体标记和活细胞成像的水溶性多功能探针

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-11-04 Epub Date: 2024-09-12 DOI:10.1002/cbic.202400377
Carmela Bonaccorso, Lorena Maria Cucci, Vanessa Sanfilippo, Cristina Munzone, Cosimo G Fortuna, Cristina Satriano
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引用次数: 0

摘要

我们报告了一种水溶性荧光比色铜探针(LysoBC1);该系统还可用于溶酶体标记和活细胞中 Cu2+ 的动态跟踪。其传感机制利用了以下三个部分的协同作用:i) 溶酶体靶向单元;ii) 选择性铜螯合的螺内酰胺开环;iii) 金属介导的罗丹明分子水解以增强荧光。在水环境中,该分子可作为荧光可逆 pH 值传感器,并在生理 pH 值下作为 Cu2+ 的比色探针;铜靶向单元的水解可使荧光强度增加 50 倍。最重要的是,通过对未分化(SH SY5Y)和已分化(d-SH SY5Y)的神经母细胞瘤细胞进行体外细胞分析,LysoBC1 能够选择性地积聚到溶酶体中,而铜的结合能力使我们能够监测细胞内铜在溶酶体中的积聚情况。
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A Water-Soluble Multifunctional Probe for Colorimetric Copper Sensing, Lysosome Labelling and Live-Cell Imaging.

We report a water-soluble fluorescence and colorimetric copper probe (LysoBC1); this system can also serve for lysosome labeling and for the dynamic tracking of Cu2+ in living cells. The sensing mechanism takes advantage of the synergic action by the following three components: i) a lysosome targeting unit, ii) the spirolactam ring-opening for the selective copper chelation and iii) the metal-mediated hydrolysis of the rhodamine moiety for fluorescence enhancement. In aqueous environment the molecule acts as a fluorescent reversible pH sensor and as colorimetric probe for Cu2+ at physiological pH; the hydrolysis of the copper targeting unit resulted in a 50-fold increase of the fluorescence intensity. Most importantly, in vitro cell analyses in undifferentiated (SH SY5Y) and differentiated (d-SH SY5Y) neuroblastoma cells, LysoBC1 is able to selectively accumulate into lysosome while the copper binding ability allowed us to monitor intracellular copper accumulation into lysosome.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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