基于萘二甲酰亚胺的单色团发射比率荧光传感器，用于跟踪细胞内 pH 值。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-11-04 Epub Date: 2024-10-16 DOI:10.1002/cbic.202400538

Sujit Kumar Das, Smitaroopa Kahali, Sabnam Kar, Nandita Madhavan, Ankona Datta

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引用次数: 0

摘要

我们报告了一种新型、可逆、细胞渗透性 pH 传感器 TRapH。TRapH 在 pH 值 ~3-6 范围内具有 pH 值敏感的比率发射响应，可对活细胞内的 pH 值进行成像和量化。通过对合成 H+ 转运体诱导的活哺乳动物细胞内 pH 动态进行实时跟踪，说明了 TRapH 在生物学上的适用性。

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Naphthalimide-Based, Single-Chromophore, Emission Ratiometric Fluorescent Sensor for Tracking Intracellular pH.

We report a novel, reversible, cell-permeable, pH-sensor, TRapH. TRapH afforded a pH-sensitive ratiometric emission response in the pH range ~3-6, enabling imaging and quantification of pH in living cells. The biological-applicability of TRapH was illustrated via live-tracking of intracellular pH dynamics in living mammalian cells induced by a synthetic H⁺-transporter.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464