评估作为肝硬化 HCV 感染者非侵入性诊断生物标志物的纤维化相关基因的表达。

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cytokine Pub Date : 2024-07-27 DOI:10.1016/j.cyto.2024.156714
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引用次数: 0

摘要

肝硬化是一种死亡率很高的疾病,对全世界的健康和经济造成了巨大负担。肝硬化的临床特征复杂多样。因此,对肝硬化中涉及免疫浸润的基因进行评估已成为肝病研究的必修课,这不仅能确定潜在的生物标志物,还能为了解该病的潜在机制提供重要依据。本研究旨在调查 HCV 患者外周血单核细胞(PBMC)中细胞因子基因的表达谱,并确定与晚期肝硬化相关的基因表达特征。我们对90名HCV基因4型患者进行了横断面研究,包括无纤维化患者(F0,24人)、纤维化患者(F1-F3,36人)和肝硬化患者(F4,30人)。通过实时定量 PCR 分析了受试者 PBMC 中细胞因子基因的表达情况,并通过 ELISA 检测了血清中 TGFβ2 的水平。我们的研究结果表明,与无纤维化患者相比,肝硬化和纤维化患者的 TGIF1 转录本表达水平较低(p = 0.046 和 0.022)。此外,肝硬化患者的 TGFβ1 基因比纤维化患者上调(p = 0.015)。此外,与无肝硬化或纤维化患者相比,肝硬化患者的 TGF-β2 转录物表达水平更高,TGF-β2 蛋白水平也有所升高。根据 ROC 分析,TGFβ1、TGIF1 转录本和 TGFβ2 蛋白在区分肝硬化、肝纤维化和非肝纤维化患者方面具有良好的鉴别性能。我们的研究结果表明,TGIF1、TGF-β1 和 TGF-β2 基因在 PBMCs 中的表达可为鉴别晚期肝硬化患者提供有价值的工具,TGF-β 和 TGIF1 可能是肝硬化的潜在生物标志物。这些发现可能会对 HCV 患者肝硬化的诊断和治疗产生影响。
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Evaluation of the expression of fibrosis-related genes as non-invasive diagnostic biomarkers for cirrhotic HCV-infected patients

Liver cirrhosis is a condition with high mortality that poses a significant health and economic burden worldwide. The clinical characteristics of liver cirrhosis are complex and varied. Therefore, the evaluation of immune infiltration-involved genes in cirrhosis has become mandatory in liver disease research, not only to identify the potential biomarkers but also to provide important insights into the underlying mechanisms of the disease. In this study, we aimed to investigate the expression profile of cytokine genes in peripheral blood mononuclear cells (PBMCs) of HCV patients and identify the gene expression signature associated with advanced cirrhosis. A cross-sectional study of 90 HCV genotype 4 patients, including no fibrosis patients (F0, n = 24), fibrotic patients (F1-F3, n = 36), and cirrhotic patients (F4, n = 30) has been conducted. The expression of cytokine genes was analyzed by quantitative real-time PCR in the subjects’ PBMCs, and the serum level of TGFβ2 was measured by ELISA. Our findings showed that the expression level of the TGIF1 transcript was lower in cirrhotic and fibrotic patients compared to no fibrosis patients (p = 0.046 and 0.022, respectively). Also, there was an upregulation of the TGFβ1 gene in cirrhotic patients relative to fibrotic patients (p = 0.015). Additionally, the cirrhotic patients had higher expression levels of the TGF-β2 transcript and elevated levels of the TGF-β2 protein than patients with no cirrhosis or fibrosis. According to the ROC analysis, TGFβ1, TGIF1 transcripts, and TGFβ2 protein have a good discriminatory performance in distinguishing between cirrhotic, fibrotic, and non-fibrotic patients. Our results suggested that the expression of TGIF1, TGF-β1, and TGF-β2 genes in PBMCs may provide a valuable tool for identifying patients with advanced cirrhosis and that TGF-β and TGIF1 may be potential biomarkers for cirrhosis. These findings may have implications for the diagnosis and treatment of cirrhosis in HCV patients.

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来源期刊
Cytokine
Cytokine 医学-免疫学
CiteScore
7.60
自引率
2.60%
发文量
262
审稿时长
48 days
期刊介绍: The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.
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