Abdallah S Abdelazem, Azza Ali Khalil, Dalia Ghareeb, Ahmad Sallam Soliman, Hanaa M El Maghraby, Nagwan A Ismail, Samia Hussein
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GA and AA genotypes were more frequent in the asthmatic group compared to the control group (<i>P</i> = 0.03 and 0.01, respectively). Subjects carrying GA and AA genotypes were more susceptible to have asthma [odds ratio (OR) = 2.21, 95% confidence interval (CI) = 1.14-9.94, <i>P</i> = 0.03; OR = 7.78, 95% CI = 1.59-38.3, <i>P</i> = 0.01, respectively]. The A allele was higher in the asthmatic group (33%) compared to the control group (10%). A allele carriers were more susceptible to have asthma (OR = 4.43, 95% CI = 2.04-9.82 and <i>P</i> < 0.001). Immunoglobulin E (IgE) levels and eosinophil percentages were higher among the carriers of GA and AA genotypes when compared with the GG genotype. All pulmonary function tests were significantly lower among carriers of AA genotype compared with GG genotype. An A allele carrier, AA genotype, increased IgE level, and eosinophil level were significant predictors for occurrence of asthma (<i>P</i> = 0.01, 0.02, 0.004, and 0.01). In conclusion, AA genotype carriers and A allele carriers of the <i>IL-17</i> gene are more likely to have asthma compared with controls.</p>","PeriodicalId":16261,"journal":{"name":"Journal of Interferon and Cytokine Research","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Interleukin-17</i> Genotypes in Egyptian Asthmatic Children at Zagazig University Hospitals.\",\"authors\":\"Abdallah S Abdelazem, Azza Ali Khalil, Dalia Ghareeb, Ahmad Sallam Soliman, Hanaa M El Maghraby, Nagwan A Ismail, Samia Hussein\",\"doi\":\"10.1089/jir.2024.0098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Bronchial asthma (BA) is increasing among Egyptian children. It is affected by multiple factors including genetic ones. In the current study, we assessed the relationship between <i>interleukin-17</i> (<i>IL-17</i>) genotypes and the occurrence of BA among Egyptian children. This case-control study included 100 participants. Group I (the control group) comprised 50 healthy subjects. Group II (the asthmatic group) comprised 50 subjects diagnosed with atopic asthma according to the Global Initiative for Asthma. Measurement of serum Ig E and eosinophilic count was performed. Detection of single nucleotide polymorphism <i>rs2275913 of IL-17</i> gene by restriction fragment length polymorphism-polymerase chain reaction was conducted. GA and AA genotypes were more frequent in the asthmatic group compared to the control group (<i>P</i> = 0.03 and 0.01, respectively). Subjects carrying GA and AA genotypes were more susceptible to have asthma [odds ratio (OR) = 2.21, 95% confidence interval (CI) = 1.14-9.94, <i>P</i> = 0.03; OR = 7.78, 95% CI = 1.59-38.3, <i>P</i> = 0.01, respectively]. The A allele was higher in the asthmatic group (33%) compared to the control group (10%). A allele carriers were more susceptible to have asthma (OR = 4.43, 95% CI = 2.04-9.82 and <i>P</i> < 0.001). Immunoglobulin E (IgE) levels and eosinophil percentages were higher among the carriers of GA and AA genotypes when compared with the GG genotype. All pulmonary function tests were significantly lower among carriers of AA genotype compared with GG genotype. An A allele carrier, AA genotype, increased IgE level, and eosinophil level were significant predictors for occurrence of asthma (<i>P</i> = 0.01, 0.02, 0.004, and 0.01). In conclusion, AA genotype carriers and A allele carriers of the <i>IL-17</i> gene are more likely to have asthma compared with controls.</p>\",\"PeriodicalId\":16261,\"journal\":{\"name\":\"Journal of Interferon and Cytokine Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Interferon and Cytokine Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1089/jir.2024.0098\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Interferon and Cytokine Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/jir.2024.0098","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/27 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
支气管哮喘(BA)在埃及儿童中的发病率越来越高。它受到包括遗传因素在内的多种因素的影响。在本研究中,我们评估了白细胞介素-17(IL-17)基因型与埃及儿童支气管哮喘发病率之间的关系。这项病例对照研究包括 100 名参与者。第一组(对照组)包括 50 名健康受试者。第二组(哮喘组)由 50 名根据全球哮喘倡议被诊断为特应性哮喘的受试者组成。测量血清 Ig E 和嗜酸性粒细胞计数。通过限制性片段长度多态性聚合酶链反应检测了 IL-17 基因的单核苷酸多态性 rs2275913。与对照组相比,GA 和 AA 基因型在哮喘组中更为常见(P = 0.03 和 0.01)。携带 GA 和 AA 基因型的受试者更易患哮喘[几率比(OR)= 2.21,95% 置信区间(CI)= 1.14-9.94,P = 0.03;OR = 7.78,95% CI = 1.59-38.3,P = 0.01]。与对照组(10%)相比,哮喘组的 A 等位基因携带者比例更高(33%)。A 等位基因携带者更容易患哮喘(OR = 4.43,95% CI = 2.04-9.82,P < 0.001)。与 GG 基因型相比,GA 和 AA 基因型携带者的免疫球蛋白 E(IgE)水平和嗜酸性粒细胞百分比更高。与 GG 基因型相比,AA 基因型携带者的所有肺功能测试结果均明显降低。A 等位基因携带者、AA 基因型、IgE 水平升高和嗜酸性粒细胞水平升高是哮喘发生的重要预测因素(P = 0.01、0.02、0.004 和 0.01)。总之,与对照组相比,IL-17 基因的 AA 基因型携带者和 A 等位基因携带者更容易患哮喘。
Interleukin-17 Genotypes in Egyptian Asthmatic Children at Zagazig University Hospitals.
Bronchial asthma (BA) is increasing among Egyptian children. It is affected by multiple factors including genetic ones. In the current study, we assessed the relationship between interleukin-17 (IL-17) genotypes and the occurrence of BA among Egyptian children. This case-control study included 100 participants. Group I (the control group) comprised 50 healthy subjects. Group II (the asthmatic group) comprised 50 subjects diagnosed with atopic asthma according to the Global Initiative for Asthma. Measurement of serum Ig E and eosinophilic count was performed. Detection of single nucleotide polymorphism rs2275913 of IL-17 gene by restriction fragment length polymorphism-polymerase chain reaction was conducted. GA and AA genotypes were more frequent in the asthmatic group compared to the control group (P = 0.03 and 0.01, respectively). Subjects carrying GA and AA genotypes were more susceptible to have asthma [odds ratio (OR) = 2.21, 95% confidence interval (CI) = 1.14-9.94, P = 0.03; OR = 7.78, 95% CI = 1.59-38.3, P = 0.01, respectively]. The A allele was higher in the asthmatic group (33%) compared to the control group (10%). A allele carriers were more susceptible to have asthma (OR = 4.43, 95% CI = 2.04-9.82 and P < 0.001). Immunoglobulin E (IgE) levels and eosinophil percentages were higher among the carriers of GA and AA genotypes when compared with the GG genotype. All pulmonary function tests were significantly lower among carriers of AA genotype compared with GG genotype. An A allele carrier, AA genotype, increased IgE level, and eosinophil level were significant predictors for occurrence of asthma (P = 0.01, 0.02, 0.004, and 0.01). In conclusion, AA genotype carriers and A allele carriers of the IL-17 gene are more likely to have asthma compared with controls.
期刊介绍:
Journal of Interferon & Cytokine Research (JICR) provides the latest groundbreaking research on all aspects of IFNs and cytokines. The Journal delivers current findings on emerging topics in this niche community, including the role of IFNs in the therapy of diseases such as multiple sclerosis, the understanding of the third class of IFNs, and the identification and function of IFN-inducible genes.