{"title":"土耳其 IBDV 分离物的分子特征发现了重交株。","authors":"G A Kurtbeyoğlu, M Akan","doi":"10.1080/00071668.2024.2379950","DOIUrl":null,"url":null,"abstract":"<p><p>1. Infectious bursal disease (IBD) is an acute, highly contagious viral disease of chickens caused by a virus (IBDV) which has a bi-segmented, double-stranded RNA genome. It has five viral proteins in its structure; the VP1 gene is encoded in segment B and the other four are in segment A.2. In this study, bursae of Fabricius and spleen samples taken from chickens suspected of having clinical or subclinical IBD from a total of 50 chicken flocks located in different geographical regions of Turkey were examined.3. The RT-PCR analysis of the VP2 gene showed that 30 of the 50 samples (60%) tested positive. Eight positive isolates were chosen and RT-PCR was performed to amplify the VP1 gene.4. The study showed that reassortant field strains that cause clinical or subclinical disease are currently circulating in broiler flocks across Turkey.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterisation of IBDV isolates in Turkey revealed reassortant strains.\",\"authors\":\"G A Kurtbeyoğlu, M Akan\",\"doi\":\"10.1080/00071668.2024.2379950\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>1. Infectious bursal disease (IBD) is an acute, highly contagious viral disease of chickens caused by a virus (IBDV) which has a bi-segmented, double-stranded RNA genome. It has five viral proteins in its structure; the VP1 gene is encoded in segment B and the other four are in segment A.2. In this study, bursae of Fabricius and spleen samples taken from chickens suspected of having clinical or subclinical IBD from a total of 50 chicken flocks located in different geographical regions of Turkey were examined.3. The RT-PCR analysis of the VP2 gene showed that 30 of the 50 samples (60%) tested positive. Eight positive isolates were chosen and RT-PCR was performed to amplify the VP1 gene.4. The study showed that reassortant field strains that cause clinical or subclinical disease are currently circulating in broiler flocks across Turkey.</p>\",\"PeriodicalId\":9322,\"journal\":{\"name\":\"British Poultry Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-07-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/00071668.2024.2379950\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00071668.2024.2379950","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
1.传染性法氏囊病（IBD）是一种由病毒（IBDV）引起的鸡的急性、高度传染性病毒性疾病。它的结构中有五种病毒蛋白，VP1 基因在 B 段编码，其他四种在 A 段编码2。3. VP2 基因的 RT-PCR 分析显示，50 个样本中有 30 个（60%）呈阳性。4. 研究表明，可引起临床或亚临床疾病的野外再变种毒株目前正在土耳其各地的肉鸡群中流行。
Molecular characterisation of IBDV isolates in Turkey revealed reassortant strains.
1. Infectious bursal disease (IBD) is an acute, highly contagious viral disease of chickens caused by a virus (IBDV) which has a bi-segmented, double-stranded RNA genome. It has five viral proteins in its structure; the VP1 gene is encoded in segment B and the other four are in segment A.2. In this study, bursae of Fabricius and spleen samples taken from chickens suspected of having clinical or subclinical IBD from a total of 50 chicken flocks located in different geographical regions of Turkey were examined.3. The RT-PCR analysis of the VP2 gene showed that 30 of the 50 samples (60%) tested positive. Eight positive isolates were chosen and RT-PCR was performed to amplify the VP1 gene.4. The study showed that reassortant field strains that cause clinical or subclinical disease are currently circulating in broiler flocks across Turkey.
期刊介绍：
From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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