通过 RNA 干扰靶向 Syntaxin 1A 可抑制蝗虫的摄食和中肠发育。

IF 2.9 1区 农林科学 Q1 ENTOMOLOGY Insect Science Pub Date : 2024-07-29 DOI:10.1111/1744-7917.13419
Xiaojian Liu, Ya Gao, Yao Li, Jianzhen Zhang
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引用次数: 0

摘要

合成轴突蛋白 1A(Syx1A)在动物体内具有多种不可或缺的功能。以前的研究主要集中在果蝇体内 Syx1A 的作用上,因此对 Syx1A 在其他昆虫发育过程中的作用仍然知之甚少。本研究调查了利用 RNA 干扰(RNAi)破坏 LmSyx1A 是否会影响蝗虫的生长发育。LmSyx1A 在测试的所有组织中均有表达,其中脂肪体的表达量最高。给5龄若虫注射双链LmSyx1A(dsLmSyx1A)后,所有若虫都无法正常蜕皮,最终全部死亡。LmSyx1A 的沉默导致蝗虫停止摄食、体重减轻、中肠和胃盲肠萎缩。血色素和伊红(H&E)染色显示,中肠的柱状细胞严重受损,注射了dsLmSyx1A的若虫可见微绒毛缺陷。透射电子显微镜(TEM)观察到分泌泡。此外,反转录定量聚合酶链反应(RT-qPCR)进一步表明,沉默 LmSyx1A 会抑制参与胰岛素/哺乳动物雷帕霉素靶标(mTOR)相关营养途径的基因的表达。综上所述,这些结果表明,LmSyx1A 对蝗虫若虫的中肠细胞层有显著影响,这与胰岛素/mTOR 相关营养途径的下调有部分关联。因此,我们认为 LmSyx1A 是开发基于 dsRNA 的生物农药来管理蝗虫的合适靶标。
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Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria.

Syntaxin 1A (Syx1A) has diverse and indispensable functions in animals. Previous studies have mainly focused on the roles of Syx1A in Drosophila, and so how Syx1A operates during the development of other insects remains poorly understood. This study investigated whether disrupting LmSyx1A using RNA interference (RNAi) affects the growth and development of Locusta migratoria. LmSyx1A was expressed in all tissues tested, with the highest expression observed in the fat body. After 5th-instar nymphs were injected with double-stranded LmSyx1A (dsLmSyx1A), none of the nymphs were able to molt normally and all eventually died. The silencing of LmSyx1A resulted in the cessation of feeding, body weight loss, and atrophy of the midgut and gastric cecum in locusts. Hematoxylin and eosin (H&E) staining showed that the columnar cells in the midgut were severely damaged, with microvilli defects visible in dsLmSyx1A-injected nymphs. Secretory vesicles were observed with transmission electron microscopy (TEM). In addition, reverse transcription quantitative polymerase chain reaction (RT-qPCR) further indicates that silencing LmSyx1A repressed the expression of genes involved in the insulin/mammalian target of rapamycin (mTOR)-associated nutritional pathway. Taken together, these results suggest that LmSyx1A significantly affects the midgut cell layer of locust nymphs, which was partially associated with the downregulation of the insulin/mTOR-associated nutritional pathway. Thus, we argue that LmSyx1A is a suitable target for developing dsRNA-based biological pesticides for managing L. migratoria.

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来源期刊
Insect Science
Insect Science 生物-昆虫学
CiteScore
7.80
自引率
5.00%
发文量
1379
审稿时长
6.0 months
期刊介绍: Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
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