Jinghua Bu, Minjie Zhang, Rongrong Zhang, Le Sun, Zhenzong Chen, Yang Wu
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引用次数: 0
摘要
本研究旨在调查眼腺中 ACE2 的表达水平，并研究 S 蛋白对眼腺的影响。实验使用雄性 C57BL/6J 小鼠。ACE2在睑板腺的表达水平最高，其次是结膜、角膜和泪腺。共免疫共沉淀试验证实，眼表上皮和睑板腺中的 ACE2 与 S 蛋白直接结合。在 S 蛋白处理组中发现了 CD45+ 细胞浸润，并伴随着炎症相关细胞因子的上调。S 蛋白处理组的细胞凋亡也很明显。总之，不仅角膜和结膜，睑板腺也表达 ACE2，S 蛋白可诱导眼表上皮细胞和睑板腺细胞炎症和凋亡。
The aim of the study was to investigate the expression levels of ACE2 in ocular glands and to investigate the effect of S protein on them. Male C57BL/6J mice were used for the experiments. The expression levels of ACE2 are highest in the Meibomian glands, followed by the conjunctiva, the cornea, and the lacrimal glands. Co-immunoprecipitation assays confirmed direct binding between ACE2 and S protein in ocular surface epithelia and Meibomian glands. CD45+ cell infiltration was found in the S protein treatment group, which was accompanied by upregulation of inflammation-related cytokines. There was also prominent cell apoptosis in the S protein treatment group. In conclusion, not only the cornea and the conjunctiva, but also the Meibomian glands express ACE2, and S protein could induce ocular surface epithelial cell and Meibomian gland cell inflammation and apoptosis.
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