B. Karlsen, Dhurba Adhikari, T. E. Jørgensen, I. K. Hanssen, T. Moum, J. T. Nordeide, Steinar Daae Johansen
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引用次数: 0
摘要
挪威的一条亚北极水道因其三刺鱼群(Gasterosteus aculeatus Linnaeus 1758)具有不寻常的骨盆脊柱多态性而闻名;上游湖泊中混有正常脊柱、不对称脊柱和无脊柱的标本。相比之下,下游湖泊和附近海域的标本只有正常脊柱形态。我们利用有丝分裂基因组学研究了水道中的母系遗传结构。我们对代表两个湖泊和一个海域的 242 条棍尾鱼进行了个体和集合 DNA 测序评估。在上游湖泊(Storvatnet 湖)中发现了两个不同的有丝分裂基因组支系,而在下游湖泊中只发现了其中一个支系。然而,海洋站点的集合 DNA 样本中包含多个有丝分裂基因组单倍型。我们介绍了有丝分裂基因组的序列特征,包括基因特异性单核苷酸多态性、分子系统发育以及基于每个地点的集合种群样本配对比较的遗传分化评估。Storvatnet湖的有丝分裂基因组属于两个不同的欧洲-北美(ENA)支系;其中一个祖系可能与水道中的原始母系相对应,而另一个祖系则是最近引入的。我们推测,第二个入侵者携带的核基因组特征导致了今天在 Storvatnet 湖观察到的骨盆脊柱多态性。
Two Distinct Maternal Lineages of Threespine Stickleback (Gasterosteus aculeatus) in a Small Norwegian Subarctic Lake
A subarctic Norwegian watercourse is known for its population of threespine sticklebacks (Gasterosteus aculeatus Linnaeus 1758) with unusual pelvic spine polymorphism; the upper lake contains a mixture of specimens that are normal-spined, asymmetric-spined, and spineless. In contrast, the downstream lakes and the nearby marine site contain only the normal spined morph. We investigated the maternal-line genetic structure in the watercourse using mitogenomics. A total of 242 sticklebacks representing two lakes and a marine site were assessed based on individual and pooled DNA sequencing. While two distinct mitogenome clades were detected in the upper lake (Lake Storvatnet), only one of these clades was present in the downstream lake. The marine site pooled DNA sample, however, contained several mitogenome haplotypes. We present mitogenome sequence features that include gene-specific single nucleotide polymorphisms, molecular phylogeny, and genetic differentiation assessments based on pairwise comparisons of pooled population samples from each site. The Lake Storvatnet mitogenomes belong to two distinct Euro-North American (ENA) clades; one of the ancestral lineages likely corresponds to the original maternal lineage in the watercourse, and the other was introduced more recently. We hypothesize that the second invader carried nuclear genomic features responsible for the observed present-day pelvic spine polymorphism in Lake Storvatnet.