S. Selim, Yasir S. Alruwaili, Emad Manni, Muhammad Atif, M. Almuhayawi, M. Alruhaili, M. A. Bazuhair, Eman M Abdelkareem, Badriah Saleh Alammari, S. A. Al Jaouni
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引用次数: 0
摘要
众所周知,植物代谢物的功效取决于其萃取方法。在对蔓荆子叶进行超临界流体萃取(SFE)后,对萃取物中植物成分的产量和组成进行了评估:静态萃取时间(SET)为 30 分钟,随后动态萃取时间(DET)为 30 分钟(条件 A);静态萃取时间为 0 分钟,随后动态萃取时间为 60 分钟(条件 B)。高效液相色谱分析显示,B 条件下的提取物中含有高浓度的肉桂酸、山柰酚、阿魏酸、芦丁和咖啡酸等化合物。金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌、粪肠球菌和白色念珠菌受条件 B 的影响较大,抑制区分别为 32 ± 0.1、20 ± 0.2、32 ± 0.2、35 ± 0.2 和 40 ± 0.1 毫米。与条件 A 相比,暴露于条件 B 的提取物对白念球菌和细菌的 MIC 和 MBC 更低。暴露于 B 条件下的提取物对卵巢肿瘤细胞的 IC50 值较高。目前,暴露于 B 条件下的提取物对伤口愈合过程的功效已得到证实。
Dynamic extraction time’s effect on phytochemical characterization of Vitex agnus-castus dry biomass with healing properties and their activity against microorganisms and ovarian cancer
Efficacies of plant metabolites are known to be dependent on their extraction methods. Yields and compositions of phytoconstituents in the extract were evaluated following supercritical fluid extraction (SFE) of Vitex agnus-castus leaves, static extraction times (SET) for 30 min, subsequently dynamic extraction time (DET) for 30 min (condition A) and SET for 0 min followed by DET for 60 min (condition B). The extract exposed to condition B gave an extraction yield of 0.169 g compared to 0.115 g for condition A. High-performance liquid chromatography analysis revealed compounds including cinnamic acid, kaempferol, ferulic acid, rutin, and caffeic acid, in high concentrations in the extract exposed to condition B. Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus faecalis, and Candida albicans were more affected by the condition B with 32 ± 0.1, 20 ± 0.2, 32 ± 0.2, 35 ± 0.2, and 40 ± 0.1 mm inhibition zones, respectively. Less MIC and MBC were noticed of the exposed extract to condition B than to condition A against C. albicans and bacteria. The IC50 of the extract exposed to condition B was high against ovarian tumor cells. Presently the efficacy of the exposed extract to condition B for wound healing process was documented.
期刊介绍:
The purpose of BioResources is to promote scientific discourse and to foster scientific developments related to sustainable manufacture involving lignocellulosic or woody biomass resources, including wood and agricultural residues. BioResources will focus on advances in science and technology. Emphasis will be placed on bioproducts, bioenergy, papermaking technology, wood products, new manufacturing materials, composite structures, and chemicals derived from lignocellulosic biomass.