揭示抗氧化剂与抗癌剂的协同作用:利用 HeLa 细胞系探索椭圆茜草和白苧麻甲醇提取物中的治疗生物活性物质

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS Assay and drug development technologies Pub Date : 2024-10-01 Epub Date: 2024-07-31 DOI:10.1089/adt.2024.040
Vipresh Bhardwaj, G T Kulkarni, Kalpana Nagpal
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引用次数: 0

摘要

本研究旨在评估从椭圆茜草(Rubus ellipticus)和白苧(Boerhavia diffusa)果实中提取的甲醇提取物对 HeLa 细胞系的协同抗氧化和抗癌作用。从椭圆茜和白苧的果实中提取甲醇提取物,并使用 2,2-二苯基-1-苦基肼(DPPH)清除活性测定法和铁还原抗氧化力(FRAP)测定法评估其抗氧化潜力。此外,还使用 3-(4,5-二甲基噻唑基-2)-2,5-二苯基溴化四唑测定法来测量细胞代谢活性,从而研究了从上述水果中提取的苯甲酸和芦丁对 HeLa 细胞系的抗癌作用。利用 Synergy Finder plus 软件,对生物活性化合物进行了测试,以探索是否存在协同效应。与 B. diffusa 相比,R. ellipticus 具有更高的 DPPH 清除活性和 FRAP 值,表现出更高的抗氧化潜力。从 R. ellipticus 中提取的苯甲酸对 HeLa 细胞系具有很强的抗癌活性,其 IC50 值为 1.07 µg/mL。同样,从白花蛇舌草中提取的芦丁也显示出中等程度的抗癌活性,其 IC50 值为 1.4 µg/mL,而对正常细胞系的影响则微乎其微。对提取的生物活性化合物进行的组合研究显示了协同效应。这些研究结果表明,R. ellipticus 和 B. diffusa 在对抗氧化应激和癌症方面具有治疗潜力。据观察,苯甲酸和芦丁等生物活性化合物具有协同作用。还需要进一步研究以阐明其潜在机制,并评估它们在临床环境中的适用性。
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Unlocking Antioxidant-Anticancer Synergy: An Exploration of Therapeutic Bioactives from Methanolic Extracts of Rubus ellipticus and Boerhavia diffusa Using HeLa Cell Line.

This study aimed to assess the synergistic antioxidant and anticancer effects of methanolic extracts derived from Rubus ellipticus and Boerhavia diffusa fruits against the HeLa cell line. The methanolic extracts were prepared from the fruits of R. ellipticus and B. diffusa, and their antioxidant potential was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity assay and the ferric reducing antioxidant power (FRAP) assay. The anticancer effects of benzoic acid and rutin extracted from the aforementioned fruits were also investigated against the HeLa cell line using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay to measure the cell metabolic activity. Using Synergy Finder plus software, the bioactive compounds were tested to explore any synergistic effects. R. ellipticus exhibited higher antioxidant potential as revealed by higher DPPH scavenging activity and FRAP value compared with B. diffusa. The benzoic acid extracted from R. ellipticus demonstrated potent anticancer activity against the HeLa cell line, with an IC50 of 1.07 µg/mL. Similarly, rutin extracted from B. diffusa displayed moderate anticancer activity with an IC50 of 1.4 µg/mL while exhibiting minimal impact on normal cell lines. The combination studies of the extracted bioactive compounds revealed a synergistic effect. These findings suggest the therapeutic potential of R. ellipticus and B. diffusa in combating the oxidative stress and cancer. Their bioactive compounds like benzoic acid and rutin were observed to act synergistically. Further investigations are warranted to elucidate the underlying mechanisms and evaluate their applicability in clinical settings.

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来源期刊
Assay and drug development technologies
Assay and drug development technologies 医学-生化研究方法
CiteScore
3.60
自引率
0.00%
发文量
33
审稿时长
>12 weeks
期刊介绍: ASSAY and Drug Development Technologies provides access to novel techniques and robust tools that enable critical advances in early-stage screening. This research published in the Journal leads to important therapeutics and platforms for drug discovery and development. This reputable peer-reviewed journal features original papers application-oriented technology reviews, topical issues on novel and burgeoning areas of research, and reports in methodology and technology application. ASSAY and Drug Development Technologies coverage includes: -Assay design, target development, and high-throughput technologies- Hit to Lead optimization and medicinal chemistry through preclinical candidate selection- Lab automation, sample management, bioinformatics, data mining, virtual screening, and data analysis- Approaches to assays configured for gene families, inherited, and infectious diseases- Assays and strategies for adapting model organisms to drug discovery- The use of stem cells as models of disease- Translation of phenotypic outputs to target identification- Exploration and mechanistic studies of the technical basis for assay and screening artifacts
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