土耳其葡萄园中葡萄罗丹花叶变色相关病毒的遗传多样性和种群结构

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-07-31 DOI:10.1111/jph.13368
Ç. Ulubaş-Serçe, S. Aydınlık, Z. N. Öztürk, S. Önder, O. Çiftçi, B. Altan
{"title":"土耳其葡萄园中葡萄罗丹花叶变色相关病毒的遗传多样性和种群结构","authors":"Ç. Ulubaş-Serçe,&nbsp;S. Aydınlık,&nbsp;Z. N. Öztürk,&nbsp;S. Önder,&nbsp;O. Çiftçi,&nbsp;B. Altan","doi":"10.1111/jph.13368","DOIUrl":null,"url":null,"abstract":"<p>Grapevine Roditis leaf discoloration-associated virus (GRLDaV) is a type of virus that infects grapevines. This research aimed to determine the prevalence of GRLDaV in Turkey vineyards and investigate the genetic diversity and population structure of the selected viral isolates from Turkey compared to isolates from other countries. In this study, 800 shoot samples from Turkey vineyards were collected in late spring and autumn from different regions. The samples were analysed by RT-PCR targeting the partial RNaseH-like gene region of the virus genome. The overall prevalence of GRLDaV was 8.1% among the samples. Phylogenetic analysis of the GRLDaV genome showed that the GRLDaV isolates were clustered into two major lineages. The nucleotide diversity (π) value of Group I, including only Mediterranean isolates, was lower than Group II, suggesting little variation among isolates. Even though π values were low among the geographical regions in Turkey, the region with the highest nucleotide diversity was the Aegean population, followed by the Eastern and Mediterranean populations. Comparison between nonsynonymous and synonymous substitutions showed that negative selection acts on synonymous amino acids. Along with neutrality tests and gene flow analyses, it was emphasized that genetic drift played an important role in shaping the GRLDaV populations. This study provides important information on the prevalence and genetic diversity of GRLDaV in Turkey vineyards. The findings will help to develop better strategies to manage and control this virus, which can cause significant economic losses in grape industries.</p>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"172 4","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic diversity and population structure of grapevine Roditis leaf discoloration-associated virus in Turkey vineyards\",\"authors\":\"Ç. Ulubaş-Serçe,&nbsp;S. Aydınlık,&nbsp;Z. N. Öztürk,&nbsp;S. Önder,&nbsp;O. Çiftçi,&nbsp;B. Altan\",\"doi\":\"10.1111/jph.13368\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Grapevine Roditis leaf discoloration-associated virus (GRLDaV) is a type of virus that infects grapevines. This research aimed to determine the prevalence of GRLDaV in Turkey vineyards and investigate the genetic diversity and population structure of the selected viral isolates from Turkey compared to isolates from other countries. In this study, 800 shoot samples from Turkey vineyards were collected in late spring and autumn from different regions. The samples were analysed by RT-PCR targeting the partial RNaseH-like gene region of the virus genome. The overall prevalence of GRLDaV was 8.1% among the samples. Phylogenetic analysis of the GRLDaV genome showed that the GRLDaV isolates were clustered into two major lineages. The nucleotide diversity (π) value of Group I, including only Mediterranean isolates, was lower than Group II, suggesting little variation among isolates. Even though π values were low among the geographical regions in Turkey, the region with the highest nucleotide diversity was the Aegean population, followed by the Eastern and Mediterranean populations. Comparison between nonsynonymous and synonymous substitutions showed that negative selection acts on synonymous amino acids. Along with neutrality tests and gene flow analyses, it was emphasized that genetic drift played an important role in shaping the GRLDaV populations. This study provides important information on the prevalence and genetic diversity of GRLDaV in Turkey vineyards. The findings will help to develop better strategies to manage and control this virus, which can cause significant economic losses in grape industries.</p>\",\"PeriodicalId\":16843,\"journal\":{\"name\":\"Journal of Phytopathology\",\"volume\":\"172 4\",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jph.13368\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13368","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

葡萄罗丹花叶变色相关病毒(GRLDaV)是一种感染葡萄藤的病毒。本研究旨在确定 GRLDaV 在土耳其葡萄园中的流行情况,并研究土耳其所选病毒分离株与其他国家分离株的遗传多样性和种群结构。这项研究在春末和秋初从土耳其不同地区的葡萄园采集了 800 份嫩枝样本。对样本进行了针对病毒基因组部分 RNaseH 样基因区域的 RT-PCR 分析。样本中 GRLDaV 的总体流行率为 8.1%。GRLDaV基因组的系统进化分析表明,GRLDaV分离株分为两大系。只包括地中海分离株的第一组的核苷酸多样性(π)值低于第二组,表明分离株之间的差异很小。尽管土耳其各地理区域的π值较低,但核苷酸多样性最高的区域是爱琴海种群,其次是东部和地中海种群。非同义替换和同义替换之间的比较表明,负选择作用于同义氨基酸。除了中性检验和基因流分析外,研究还强调遗传漂变在 GRLDaV 种群的形成过程中发挥了重要作用。这项研究为了解土耳其葡萄园中 GRLDaV 的流行情况和遗传多样性提供了重要信息。研究结果将有助于制定更好的策略来管理和控制这种会给葡萄产业造成重大经济损失的病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Genetic diversity and population structure of grapevine Roditis leaf discoloration-associated virus in Turkey vineyards

Grapevine Roditis leaf discoloration-associated virus (GRLDaV) is a type of virus that infects grapevines. This research aimed to determine the prevalence of GRLDaV in Turkey vineyards and investigate the genetic diversity and population structure of the selected viral isolates from Turkey compared to isolates from other countries. In this study, 800 shoot samples from Turkey vineyards were collected in late spring and autumn from different regions. The samples were analysed by RT-PCR targeting the partial RNaseH-like gene region of the virus genome. The overall prevalence of GRLDaV was 8.1% among the samples. Phylogenetic analysis of the GRLDaV genome showed that the GRLDaV isolates were clustered into two major lineages. The nucleotide diversity (π) value of Group I, including only Mediterranean isolates, was lower than Group II, suggesting little variation among isolates. Even though π values were low among the geographical regions in Turkey, the region with the highest nucleotide diversity was the Aegean population, followed by the Eastern and Mediterranean populations. Comparison between nonsynonymous and synonymous substitutions showed that negative selection acts on synonymous amino acids. Along with neutrality tests and gene flow analyses, it was emphasized that genetic drift played an important role in shaping the GRLDaV populations. This study provides important information on the prevalence and genetic diversity of GRLDaV in Turkey vineyards. The findings will help to develop better strategies to manage and control this virus, which can cause significant economic losses in grape industries.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
期刊最新文献
Nanoparticles Impact on the Morphogenetics of Cicer arietinum L. Offshoot Decline and Bud Rot of Date Palm Caused by Three Species of Enterobacteriaceae An Economic and Reliable PCR Approach to Distinguish the Primary Pathogens Armillaria mellea and A. ostoyae From Other European Armillaria Species Isolation and Identification of Pathogenic Microorganisms Causing Post-Harvest Disease of Garlic (Allium sativum L.) in Ninh Thuan Province, Vietnam Severity and Reaction to Bacterial Spot of Tomato Hybrids For Industrial Processing
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1