Adrian L. Cookson, Meg Devane, Jonathan C. Marshall, Marie Moinet, Amanda Gardner, Rose M. Collis, Lynn Rogers, Patrick J. Biggs, Anthony B. Pita, Angela J. Cornelius, Iain Haysom, David T. S. Hayman, Brent J. Gilpin, Margaret Leonard
{"title":"淡水中大肠埃希氏菌的种群结构和病原体相互作用:土地使用对新西兰奥特亚罗瓦水质和公共卫生的影响。","authors":"Adrian L. Cookson, Meg Devane, Jonathan C. Marshall, Marie Moinet, Amanda Gardner, Rose M. Collis, Lynn Rogers, Patrick J. Biggs, Anthony B. Pita, Angela J. Cornelius, Iain Haysom, David T. S. Hayman, Brent J. Gilpin, Margaret Leonard","doi":"10.1111/1758-2229.13319","DOIUrl":null,"url":null,"abstract":"<p>Freshwater samples (<i>n</i> = 199) were obtained from 41 sites with contrasting land-uses (avian, low impact, dairy, urban, sheep and beef, and mixed sheep, beef and dairy) and the <i>E. coli</i> phylotype of 3980 isolates (20 per water sample enrichment) was determined. Eight phylotypes were identified with B1 (48.04%), B2 (14.87%) and A (14.79%) the most abundant. <i>Escherichia marmotae</i> (<i>n</i> = 22), and <i>Escherichia ruysiae</i> (<i>n</i> = 1), were rare (0.68%) suggesting that these environmental strains are unlikely to confound water quality assessments. Phylotypes A and B1 were overrepresented in dairy and urban sites (<i>p</i> < 0.0001), whilst B2 were overrepresented in low impact sites (<i>p</i> < 0.0001). Pathogens ((<i>Salmonella</i>, <i>Campylobacter</i>, <i>Cryptosporidium</i> or <i>Giardia</i>) and the presence of diarrhoeagenic <i>E. coli</i>-associated genes (<i>stx</i> and <i>eae</i>) were detected in 89.9% (179/199) samples, including 80.5% (33/41) of samples with putative non-recent faecal inputs. Quantitative PCR to detect microbial source tracking targets from human, ruminant and avian contamination were concordant with land-use type and <i>E. coli</i> phylotype abundance. This study demonstrated that a potential recreational health risk remains where pathogens occurred in water samples with low <i>E. coli</i> concentration, potential non-recent faecal sources, low impact sites and where human, ruminant and avian faecal sources were absent.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 4","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11297283/pdf/","citationCount":"0","resultStr":"{\"title\":\"Population structure and pathogen interaction of Escherichia coli in freshwater: Implications of land-use for water quality and public health in Aotearoa New Zealand\",\"authors\":\"Adrian L. Cookson, Meg Devane, Jonathan C. Marshall, Marie Moinet, Amanda Gardner, Rose M. Collis, Lynn Rogers, Patrick J. Biggs, Anthony B. Pita, Angela J. Cornelius, Iain Haysom, David T. S. Hayman, Brent J. Gilpin, Margaret Leonard\",\"doi\":\"10.1111/1758-2229.13319\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Freshwater samples (<i>n</i> = 199) were obtained from 41 sites with contrasting land-uses (avian, low impact, dairy, urban, sheep and beef, and mixed sheep, beef and dairy) and the <i>E. coli</i> phylotype of 3980 isolates (20 per water sample enrichment) was determined. Eight phylotypes were identified with B1 (48.04%), B2 (14.87%) and A (14.79%) the most abundant. <i>Escherichia marmotae</i> (<i>n</i> = 22), and <i>Escherichia ruysiae</i> (<i>n</i> = 1), were rare (0.68%) suggesting that these environmental strains are unlikely to confound water quality assessments. Phylotypes A and B1 were overrepresented in dairy and urban sites (<i>p</i> < 0.0001), whilst B2 were overrepresented in low impact sites (<i>p</i> < 0.0001). Pathogens ((<i>Salmonella</i>, <i>Campylobacter</i>, <i>Cryptosporidium</i> or <i>Giardia</i>) and the presence of diarrhoeagenic <i>E. coli</i>-associated genes (<i>stx</i> and <i>eae</i>) were detected in 89.9% (179/199) samples, including 80.5% (33/41) of samples with putative non-recent faecal inputs. Quantitative PCR to detect microbial source tracking targets from human, ruminant and avian contamination were concordant with land-use type and <i>E. coli</i> phylotype abundance. This study demonstrated that a potential recreational health risk remains where pathogens occurred in water samples with low <i>E. coli</i> concentration, potential non-recent faecal sources, low impact sites and where human, ruminant and avian faecal sources were absent.</p>\",\"PeriodicalId\":163,\"journal\":{\"name\":\"Environmental Microbiology Reports\",\"volume\":\"16 4\",\"pages\":\"\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11297283/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Microbiology Reports\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.13319\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.13319","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Population structure and pathogen interaction of Escherichia coli in freshwater: Implications of land-use for water quality and public health in Aotearoa New Zealand
Freshwater samples (n = 199) were obtained from 41 sites with contrasting land-uses (avian, low impact, dairy, urban, sheep and beef, and mixed sheep, beef and dairy) and the E. coli phylotype of 3980 isolates (20 per water sample enrichment) was determined. Eight phylotypes were identified with B1 (48.04%), B2 (14.87%) and A (14.79%) the most abundant. Escherichia marmotae (n = 22), and Escherichia ruysiae (n = 1), were rare (0.68%) suggesting that these environmental strains are unlikely to confound water quality assessments. Phylotypes A and B1 were overrepresented in dairy and urban sites (p < 0.0001), whilst B2 were overrepresented in low impact sites (p < 0.0001). Pathogens ((Salmonella, Campylobacter, Cryptosporidium or Giardia) and the presence of diarrhoeagenic E. coli-associated genes (stx and eae) were detected in 89.9% (179/199) samples, including 80.5% (33/41) of samples with putative non-recent faecal inputs. Quantitative PCR to detect microbial source tracking targets from human, ruminant and avian contamination were concordant with land-use type and E. coli phylotype abundance. This study demonstrated that a potential recreational health risk remains where pathogens occurred in water samples with low E. coli concentration, potential non-recent faecal sources, low impact sites and where human, ruminant and avian faecal sources were absent.
期刊介绍:
The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side.
Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following:
the structure, activities and communal behaviour of microbial communities
microbial community genetics and evolutionary processes
microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors
microbes in the tree of life, microbial diversification and evolution
population biology and clonal structure
microbial metabolic and structural diversity
microbial physiology, growth and survival
microbes and surfaces, adhesion and biofouling
responses to environmental signals and stress factors
modelling and theory development
pollution microbiology
extremophiles and life in extreme and unusual little-explored habitats
element cycles and biogeochemical processes, primary and secondary production
microbes in a changing world, microbially-influenced global changes
evolution and diversity of archaeal and bacterial viruses
new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.