Ping Guo, Xueqin Gao, Anna-Laura Nelson, Matthieu Huard, Aiping Lu, William Sealy Hambright, Johnny Huard
{"title":"肿瘤坏死因子α诱导的类 8 蛋白 2 (TIPE2) 基因转移通过减少炎症和细胞衰老,改善了早衰小鼠模型中与衰老相关的骨关节炎。","authors":"Ping Guo, Xueqin Gao, Anna-Laura Nelson, Matthieu Huard, Aiping Lu, William Sealy Hambright, Johnny Huard","doi":"10.1016/j.ymthe.2024.07.027","DOIUrl":null,"url":null,"abstract":"<p><p>Osteoarthritis (OA) pain is often associated with the expression of tumor necrosis factor alpha (TNF-α), suggesting that TNF-α is one of the main contributing factors that cause inflammation, pain, and OA pathology. Thus, inhibition of TNF-α could potentially improve OA symptoms and slow disease progression. Anti-TNF-α treatments with antibodies, however, require multiple treatments and cannot entirely block TNF-α. TNF-α-induced protein 8-like 2 (TIPE2) was found to regulate the immune system's homeostasis and inflammation through different mechanisms from anti-TNF-α therapies. With a single treatment of adeno-associated virus (AAV)-TIPE2 gene delivery in the accelerated aging Zmpste24<sup>-/-</sup> (Z24<sup>-/-</sup>) mouse model, we found differences in Safranin O staining intensity within the articular cartilage (AC) region of the knee between TIPE2-treated mice and control mice. The glycosaminoglycan content (orange-red) was degraded in the Z24<sup>-/-</sup> cartilage while shown to be restored in the TIPE2-treated Z24<sup>-/-</sup> cartilage. We also observed that chondrocytes in Z24<sup>-/-</sup> mice exhibited a variety of senescent-associated phenotypes. Treatment with TIPE2 decreased TNF-α-positive cells, β-galactosidase (β-gal) activity, and p16 expression seen in Z24<sup>-/-</sup> mice. Our study demonstrated that AAV-TIPE2 gene delivery effectively blocked TNF-α-induced inflammation and senescence, resulting in the prevention or delay of knee OA in our accelerated aging Z24<sup>-/-</sup> mouse model.</p>","PeriodicalId":19020,"journal":{"name":"Molecular Therapy","volume":null,"pages":null},"PeriodicalIF":12.1000,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"TIPE2 gene transfer ameliorates aging-associated osteoarthritis in a progeria mouse model by reducing inflammation and cellular senescence.\",\"authors\":\"Ping Guo, Xueqin Gao, Anna-Laura Nelson, Matthieu Huard, Aiping Lu, William Sealy Hambright, Johnny Huard\",\"doi\":\"10.1016/j.ymthe.2024.07.027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Osteoarthritis (OA) pain is often associated with the expression of tumor necrosis factor alpha (TNF-α), suggesting that TNF-α is one of the main contributing factors that cause inflammation, pain, and OA pathology. Thus, inhibition of TNF-α could potentially improve OA symptoms and slow disease progression. Anti-TNF-α treatments with antibodies, however, require multiple treatments and cannot entirely block TNF-α. TNF-α-induced protein 8-like 2 (TIPE2) was found to regulate the immune system's homeostasis and inflammation through different mechanisms from anti-TNF-α therapies. With a single treatment of adeno-associated virus (AAV)-TIPE2 gene delivery in the accelerated aging Zmpste24<sup>-/-</sup> (Z24<sup>-/-</sup>) mouse model, we found differences in Safranin O staining intensity within the articular cartilage (AC) region of the knee between TIPE2-treated mice and control mice. The glycosaminoglycan content (orange-red) was degraded in the Z24<sup>-/-</sup> cartilage while shown to be restored in the TIPE2-treated Z24<sup>-/-</sup> cartilage. We also observed that chondrocytes in Z24<sup>-/-</sup> mice exhibited a variety of senescent-associated phenotypes. Treatment with TIPE2 decreased TNF-α-positive cells, β-galactosidase (β-gal) activity, and p16 expression seen in Z24<sup>-/-</sup> mice. Our study demonstrated that AAV-TIPE2 gene delivery effectively blocked TNF-α-induced inflammation and senescence, resulting in the prevention or delay of knee OA in our accelerated aging Z24<sup>-/-</sup> mouse model.</p>\",\"PeriodicalId\":19020,\"journal\":{\"name\":\"Molecular Therapy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":12.1000,\"publicationDate\":\"2024-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Therapy\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ymthe.2024.07.027\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Therapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ymthe.2024.07.027","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/5 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
骨关节炎(OA)疼痛往往与肿瘤坏死因子(TNF-α)的表达有关,这表明TNF-α是导致炎症、疼痛和OA病变的主要因素之一。因此,抑制 TNF-α 有可能改善 OA 症状,减缓疾病进展。然而,使用抗体抗TNF-α治疗需要多次治疗,而且不能完全阻断TNF-α。研究发现,肿瘤坏死因子α诱导的类8蛋白2(TIPE2)通过与抗肿瘤坏死因子α疗法不同的机制调节免疫系统的平衡和炎症。通过在加速衰老的 Z24-/- 小鼠模型中进行 AAV-TIPE2 基因递送单次治疗,我们发现 TIPE2 治疗小鼠与对照组小鼠膝关节 AC 区的 Safranin O 染色强度存在差异。Z24-/-小鼠软骨中的糖胺聚糖含量(橙红色)有所下降,而经 TIPE2 处理的 Z24-/- 小鼠软骨中的糖胺聚糖含量则有所恢复。我们还观察到,Z24-/-小鼠的软骨细胞表现出多种衰老相关表型。用 TIPE2 处理 Z24-/- 小鼠可减少 TNF-α 阳性细胞、β-Gal 活性和 p16 表达。我们的研究表明,AAV-TIPE2基因递送能有效阻断TNF-α诱导的炎症和衰老,从而预防或延缓加速衰老Z24-/-小鼠模型的膝关节OA。
TIPE2 gene transfer ameliorates aging-associated osteoarthritis in a progeria mouse model by reducing inflammation and cellular senescence.
Osteoarthritis (OA) pain is often associated with the expression of tumor necrosis factor alpha (TNF-α), suggesting that TNF-α is one of the main contributing factors that cause inflammation, pain, and OA pathology. Thus, inhibition of TNF-α could potentially improve OA symptoms and slow disease progression. Anti-TNF-α treatments with antibodies, however, require multiple treatments and cannot entirely block TNF-α. TNF-α-induced protein 8-like 2 (TIPE2) was found to regulate the immune system's homeostasis and inflammation through different mechanisms from anti-TNF-α therapies. With a single treatment of adeno-associated virus (AAV)-TIPE2 gene delivery in the accelerated aging Zmpste24-/- (Z24-/-) mouse model, we found differences in Safranin O staining intensity within the articular cartilage (AC) region of the knee between TIPE2-treated mice and control mice. The glycosaminoglycan content (orange-red) was degraded in the Z24-/- cartilage while shown to be restored in the TIPE2-treated Z24-/- cartilage. We also observed that chondrocytes in Z24-/- mice exhibited a variety of senescent-associated phenotypes. Treatment with TIPE2 decreased TNF-α-positive cells, β-galactosidase (β-gal) activity, and p16 expression seen in Z24-/- mice. Our study demonstrated that AAV-TIPE2 gene delivery effectively blocked TNF-α-induced inflammation and senescence, resulting in the prevention or delay of knee OA in our accelerated aging Z24-/- mouse model.
期刊介绍:
Molecular Therapy is the leading journal for research in gene transfer, vector development, stem cell manipulation, and therapeutic interventions. It covers a broad spectrum of topics including genetic and acquired disease correction, vaccine development, pre-clinical validation, safety/efficacy studies, and clinical trials. With a focus on advancing genetics, medicine, and biotechnology, Molecular Therapy publishes peer-reviewed research, reviews, and commentaries to showcase the latest advancements in the field. With an impressive impact factor of 12.4 in 2022, it continues to attract top-tier contributions.