低温(18°C)对植物病原菌 Pseudomonas savastanoi pv. phaseolicolaNPS3121 细胞内氧化应激产生的影响

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-08-01 DOI:10.1111/jph.13367
Jackeline Lizzeta Arvizu-Gómez, Alejandro Hernández-Morales, Kevin Daniel Llanos-Vargas, Gabriela Olmedo-Álvarez, Juan Campos-Guillén, Alba Adriana Vallejo-Cardona, José Luis Hernández-Flores, Christian González-Reyes
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引用次数: 0

摘要

低温是各种植物病原菌的一个关键条件,有利于它们的致病潜力和植物病害的发生。迄今为止,人们对植物病原菌与低温有关的信号转导途径知之甚少。在这种条件下发生的细胞内氧化应激被认为是属于这一信号途径的分子事件。在本研究中,我们利用沙瓦氏假单胞菌(Pseudomonas savastanoi pv. phaseolicola)模式菌评估了低温(18°C)条件下的细胞内氧化还原状态。我们利用 P. savastanoi pv. phaseolicola-roGFP2 生物报告菌株生成了氧化应激生物传感器(pKL1)并进行了荧光测定分析。在低温条件下(18°C),反应能力或 roGFP2 氧化能力相对于 28°C 有所减弱。同样,观察到 P. savastanoi pv. phaseolicola 在 28°C 和 18°C 生长期间细胞内氧化还原电位的变化,在 18°C 生长的细胞氧化程度更高。这些结果表明,低温会诱导 P. savastanoi pv. phaseolicola 细胞内的氧化应激。通过这项研究,氧化应激被确定为该细菌对低温条件的细胞反应的一部分。这是首次报道氧化应激与植物病原菌低温条件的关系。
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Influence of the low temperatures (18°C) in the generation of intracellular oxidative stress in the phytopathogen bacterium Pseudomonas savastanoi pv. phaseolicola NPS3121

Low temperatures are a key condition for various phytopathogenic bacteria, by favouring their pathogenic potential and the development of plant diseases. So far, the signal transduction pathways related to low temperatures in phytopathogenic bacteria are little understood. The occurrence of intracellular oxidative stress under this condition has been suggested as a molecular event belonging to this signalling pathway. In this study, using the Pseudomonas savastanoi pv. phaseolicola model bacteria, we evaluated intracellular redox state under low-temperature (18°C) conditions. Generation of an oxidative-stress biosensor (pKL1) and fluorometry analyses with the P. savastanoi pv. phaseolicola-roGFP2 bioreporter strain were performed. At low temperatures (18°C) the response capacity or roGFP2 oxidation diminished in relation to 28°C. Likewise, changes in the intracellular redox potential of P. savastanoi pv. phaseolicola during its growth at 28°C and 18°C were observed, with greater oxidation degree in cells grown at 18°C. These results demonstrated that low temperatures induce intracellular oxidative stress in P. savastanoi pv. phaseolicola. From this study, the oxidative stress is established as part of the cellular response to low-temperature conditions in this bacteria. This is the first report of the relation of oxidative stress with low-temperature conditions in phytopathogens bacteria.

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来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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