微流控通道和集成共面平行电极的阻抗特性是片上器官微系统全通道分析的设计参数

Biosensors Pub Date : 2024-08-01 DOI:10.3390/bios14080374
Crystal E. Rapier, Srikanth Jagadeesan, Gad D. Vatine, Hadar Ben-Yoav
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引用次数: 0

摘要

微流体技术实现了精确的物理和化学环境控制,为细胞培养带来了革命性的变化。配合电极,微流控细胞培养可以被激活或实时感知其变化。我们利用之前开发的用于细胞生长和监测的可靠、稳定的微流控装置,设计、制造并表征了一种基于全通道阻抗的传感器,并利用它系统地评估了微流控通道边界与不同的电极尺寸、距离、涂层和细胞覆盖面之间的电学和电化学影响。我们的调查包括理论和实验方法,以研究设计参数和绝缘边界条件如何改变阻抗特性。我们使用频率范围为 0.5 Hz 至 1 MHz、调制电压为 50 mV 的各种解决方案对系统进行了研究。结果表明,阻抗与电极间距成正比,与电极涂层、面积和通道大小成反比。我们还证明,电极间距是影响阻抗的主要因素。最后,我们总结了所有发现的关系,并就使用该系统研究血管模型和片上器官设备中的屏障细胞是否合适发表了评论。这项基础研究有助于精心设计需要通道几何形状和基于阻抗的生物传感的微流控培养结构和模型。
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Impedance Characteristics of Microfluidic Channels and Integrated Coplanar Parallel Electrodes as Design Parameters for Whole-Channel Analysis in Organ-on-Chip Micro-Systems
Microfluidics have revolutionized cell culture by allowing for precise physical and chemical environmental control. Coupled with electrodes, microfluidic cell culture can be activated or have its changes sensed in real-time. We used our previously developed reliable and stable microfluidic device for cell growth and monitoring to design, fabricate, and characterize a whole-channel impedance-based sensor and used it to systematically assess the electrical and electrochemical influences of microfluidic channel boundaries coupled with varying electrode sizes, distances, coatings, and cell coverage. Our investigation includes both theoretical and experimental approaches to investigate how design parameters and insulating boundary conditions change impedance characteristics. We examined the system with various solutions using a frequency range of 0.5 Hz to 1 MHz and a modulation voltage of 50 mV. The results show that impedance is directly proportional to electrode distance and inversely proportional to electrode coating, area, and channel size. We also demonstrate that electrode spacing is a dominant factor contributing to impedance. In the end, we summarize all the relationships found and comment on the appropriateness of using this system to investigate barrier cells in blood vessel models and organ-on-a-chip devices. This fundamental study can help in the careful design of microfluidic culture constructs and models that require channel geometries and impedance-based biosensing.
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