Mahmoud A. Emara, Nuha Mustafa Altilmisani, Faisal Albishri, Imtinan Akram Khan, Salah Mubark Elkhalifa, Talha A. Al-Dubai, Esam Omar Al-Wesabi
{"title":"在沙特阿拉伯吉达实地采集的埃及伊蚊中检测沃尔巴克氏体","authors":"Mahmoud A. Emara, Nuha Mustafa Altilmisani, Faisal Albishri, Imtinan Akram Khan, Salah Mubark Elkhalifa, Talha A. Al-Dubai, Esam Omar Al-Wesabi","doi":"10.1111/1748-5967.12759","DOIUrl":null,"url":null,"abstract":"<p>Recent reports have disclosed the occurrence of <i>Wolbachia</i> in <i>Aedes aegypti</i>. Our study detected <i>Wolbachia</i> infection in <i>Ae. aegypti</i> by screening wild adult mosquitoes using two <i>Wolbachia</i>-specific molecular markers. Overall, 444 adult <i>Ae. aegypti</i> mosquitoes were collected from April 2022 to October 2022 in Jeddah, Saudi Arabia. Each individual sample was processed and screened for the presence of <i>Wolbachia</i> using selected markers, the <i>Wolbachia</i>-specific <i>16S</i> rDNA and the <i>Wolbachia</i> surface protein gene (<i>wsp</i>), under optimized polymerase chain reaction (PCR) conditions, and sequenced. In total, 39 (8.78%) and 48 (10.81%) individual mosquito samples were determined to be infected with <i>Wolbachia</i> using the <i>wsp</i> and <i>16S</i> rDNA markers, respectively. By utilizing two <i>Wolbachi</i>a-specific molecular markers, our study demonstrated the presence of <i>Wolbachia</i> from individual <i>Ae. aegypti</i> samples. Our results showed a low rate of <i>Wolbachia</i> infection and inferred that the detected strain belongs to supergroup B.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 8","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Wolbachia in field-collected Aedes aegypti mosquitoes from Jeddah, Saudi Arabia\",\"authors\":\"Mahmoud A. Emara, Nuha Mustafa Altilmisani, Faisal Albishri, Imtinan Akram Khan, Salah Mubark Elkhalifa, Talha A. Al-Dubai, Esam Omar Al-Wesabi\",\"doi\":\"10.1111/1748-5967.12759\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Recent reports have disclosed the occurrence of <i>Wolbachia</i> in <i>Aedes aegypti</i>. Our study detected <i>Wolbachia</i> infection in <i>Ae. aegypti</i> by screening wild adult mosquitoes using two <i>Wolbachia</i>-specific molecular markers. Overall, 444 adult <i>Ae. aegypti</i> mosquitoes were collected from April 2022 to October 2022 in Jeddah, Saudi Arabia. Each individual sample was processed and screened for the presence of <i>Wolbachia</i> using selected markers, the <i>Wolbachia</i>-specific <i>16S</i> rDNA and the <i>Wolbachia</i> surface protein gene (<i>wsp</i>), under optimized polymerase chain reaction (PCR) conditions, and sequenced. In total, 39 (8.78%) and 48 (10.81%) individual mosquito samples were determined to be infected with <i>Wolbachia</i> using the <i>wsp</i> and <i>16S</i> rDNA markers, respectively. By utilizing two <i>Wolbachi</i>a-specific molecular markers, our study demonstrated the presence of <i>Wolbachia</i> from individual <i>Ae. aegypti</i> samples. Our results showed a low rate of <i>Wolbachia</i> infection and inferred that the detected strain belongs to supergroup B.</p>\",\"PeriodicalId\":11776,\"journal\":{\"name\":\"Entomological Research\",\"volume\":\"54 8\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Entomological Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1748-5967.12759\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1748-5967.12759","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Detection of Wolbachia in field-collected Aedes aegypti mosquitoes from Jeddah, Saudi Arabia
Recent reports have disclosed the occurrence of Wolbachia in Aedes aegypti. Our study detected Wolbachia infection in Ae. aegypti by screening wild adult mosquitoes using two Wolbachia-specific molecular markers. Overall, 444 adult Ae. aegypti mosquitoes were collected from April 2022 to October 2022 in Jeddah, Saudi Arabia. Each individual sample was processed and screened for the presence of Wolbachia using selected markers, the Wolbachia-specific 16S rDNA and the Wolbachia surface protein gene (wsp), under optimized polymerase chain reaction (PCR) conditions, and sequenced. In total, 39 (8.78%) and 48 (10.81%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. By utilizing two Wolbachia-specific molecular markers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low rate of Wolbachia infection and inferred that the detected strain belongs to supergroup B.
期刊介绍：
Entomological Research is the successor of the Korean Journal of Entomology. Published by the Entomological Society of Korea (ESK) since 1970, it is the official English language journal of ESK, and publishes original research articles dealing with any aspect of entomology. Papers in any of the following fields will be considered:
-systematics-
ecology-
physiology-
biochemistry-
pest control-
embryology-
genetics-
cell and molecular biology-
medical entomology-
apiculture and sericulture.
The Journal publishes research papers and invited reviews.
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