{"title":"三种最常见妇科恶性肿瘤瘤内微生物组的个体性和普遍性:一项观察性研究。","authors":"Qin Xiao, Wen-Jie Chen, Fei Wu, Xin-Yi Zhang, Xia Li, Jing Wei, Ting-Tao Chen, Zhao-Xia Liu","doi":"10.1128/spectrum.01004-24","DOIUrl":null,"url":null,"abstract":"<p><p>Growing evidence have indicated the crucial role of intratumor microbiome in a variety of solid tumor. However, the intratumoral microbiome in gynecological malignancies is largely unknown. In the present study, a total of 90 <i>Han</i> patients, including 30 patients with cancer in cervix, ovary, and endometrium each were enrolled, the composition of intratumoral microbiome was assessed by 16S rDNA amplicon high throughput sequencing. We found that the diversity and metabolic potential of intratumoral microbiome in all three cancer types were very similar. Furthermore, all three cancer types shared a few taxa that collectively take up high relative abundance and positive rate, including <i>Pseudomonas sp</i>., Comamonadaceae <i>gen. sp</i>., <i>Bradyrhizobium sp</i>., <i>Saccharomonospora sp</i>., <i>Cutibacterium acnes</i>, <i>Rubrobacter sp</i>., <i>Dialister micraerophilus</i>, and <i>Escherichia coli</i>. Additionally, <i>Haemophilus parainfluenzae</i> and <i>Paracoccus sp</i>. in cervical cancer, <i>Pelomonas sp</i>. in ovarian cancer, and <i>Enterococcus faecalis</i> in endometrial cancer were identified by LDA to be a representative bacterial strain. In addition, in cervical cancer patients, alpha-fetoprotein (AFP) (correlation coefficient = -0.3714) was negatively correlated (<i>r</i> = 0.4, 95% CI: 0.03 to 0.7) with <i>Rubrobacter sp</i>. and CA199 (correlation coefficient = 0.3955) was positively associated (<i>r</i> = 0.4, 95% CI: 0.03 to 0.7) with <i>Saccharomonospora sp</i>.. In ovarian cancer patients, CA125 (correlation coefficient = -0.4451) was negatively correlated (<i>r</i> = -0.4, 95% CI: -0.7 to -0.09) with <i>Porphyromonas sp.</i>. In endometrial cancer patients, CEA (correlation coefficient = -0.3868) was negatively correlated (<i>r</i> = -0.4, 95% CI: -0.7 to -0.02) with <i>Cutibacterium acnes</i>. This study promoted our understanding of the intratumoral microbiome in gynecological malignancies.IMPORTANCEIn this study, we found the compositional spectrum of tumor microbes among gynecological malignancies were largely similar by sharing a few taxa and differentiated by substantial species owned uniquely. Certain species, mostly unreported, were identified to be associated with clinical characteristics. This study prompted our understanding of gynecological malignancies and offered evidence for tumor microbes affecting tumor biology among cancers in the female reproductive system.</p>","PeriodicalId":18670,"journal":{"name":"Microbiology spectrum","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370256/pdf/","citationCount":"0","resultStr":"{\"title\":\"Individuality and generality of intratumoral microbiome in the three most prevalent gynecological malignancies: an observational study.\",\"authors\":\"Qin Xiao, Wen-Jie Chen, Fei Wu, Xin-Yi Zhang, Xia Li, Jing Wei, Ting-Tao Chen, Zhao-Xia Liu\",\"doi\":\"10.1128/spectrum.01004-24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Growing evidence have indicated the crucial role of intratumor microbiome in a variety of solid tumor. However, the intratumoral microbiome in gynecological malignancies is largely unknown. In the present study, a total of 90 <i>Han</i> patients, including 30 patients with cancer in cervix, ovary, and endometrium each were enrolled, the composition of intratumoral microbiome was assessed by 16S rDNA amplicon high throughput sequencing. We found that the diversity and metabolic potential of intratumoral microbiome in all three cancer types were very similar. Furthermore, all three cancer types shared a few taxa that collectively take up high relative abundance and positive rate, including <i>Pseudomonas sp</i>., Comamonadaceae <i>gen. sp</i>., <i>Bradyrhizobium sp</i>., <i>Saccharomonospora sp</i>., <i>Cutibacterium acnes</i>, <i>Rubrobacter sp</i>., <i>Dialister micraerophilus</i>, and <i>Escherichia coli</i>. Additionally, <i>Haemophilus parainfluenzae</i> and <i>Paracoccus sp</i>. in cervical cancer, <i>Pelomonas sp</i>. in ovarian cancer, and <i>Enterococcus faecalis</i> in endometrial cancer were identified by LDA to be a representative bacterial strain. In addition, in cervical cancer patients, alpha-fetoprotein (AFP) (correlation coefficient = -0.3714) was negatively correlated (<i>r</i> = 0.4, 95% CI: 0.03 to 0.7) with <i>Rubrobacter sp</i>. and CA199 (correlation coefficient = 0.3955) was positively associated (<i>r</i> = 0.4, 95% CI: 0.03 to 0.7) with <i>Saccharomonospora sp</i>.. In ovarian cancer patients, CA125 (correlation coefficient = -0.4451) was negatively correlated (<i>r</i> = -0.4, 95% CI: -0.7 to -0.09) with <i>Porphyromonas sp.</i>. In endometrial cancer patients, CEA (correlation coefficient = -0.3868) was negatively correlated (<i>r</i> = -0.4, 95% CI: -0.7 to -0.02) with <i>Cutibacterium acnes</i>. This study promoted our understanding of the intratumoral microbiome in gynecological malignancies.IMPORTANCEIn this study, we found the compositional spectrum of tumor microbes among gynecological malignancies were largely similar by sharing a few taxa and differentiated by substantial species owned uniquely. Certain species, mostly unreported, were identified to be associated with clinical characteristics. This study prompted our understanding of gynecological malignancies and offered evidence for tumor microbes affecting tumor biology among cancers in the female reproductive system.</p>\",\"PeriodicalId\":18670,\"journal\":{\"name\":\"Microbiology spectrum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370256/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology spectrum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1128/spectrum.01004-24\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology spectrum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1128/spectrum.01004-24","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Individuality and generality of intratumoral microbiome in the three most prevalent gynecological malignancies: an observational study.
Growing evidence have indicated the crucial role of intratumor microbiome in a variety of solid tumor. However, the intratumoral microbiome in gynecological malignancies is largely unknown. In the present study, a total of 90 Han patients, including 30 patients with cancer in cervix, ovary, and endometrium each were enrolled, the composition of intratumoral microbiome was assessed by 16S rDNA amplicon high throughput sequencing. We found that the diversity and metabolic potential of intratumoral microbiome in all three cancer types were very similar. Furthermore, all three cancer types shared a few taxa that collectively take up high relative abundance and positive rate, including Pseudomonas sp., Comamonadaceae gen. sp., Bradyrhizobium sp., Saccharomonospora sp., Cutibacterium acnes, Rubrobacter sp., Dialister micraerophilus, and Escherichia coli. Additionally, Haemophilus parainfluenzae and Paracoccus sp. in cervical cancer, Pelomonas sp. in ovarian cancer, and Enterococcus faecalis in endometrial cancer were identified by LDA to be a representative bacterial strain. In addition, in cervical cancer patients, alpha-fetoprotein (AFP) (correlation coefficient = -0.3714) was negatively correlated (r = 0.4, 95% CI: 0.03 to 0.7) with Rubrobacter sp. and CA199 (correlation coefficient = 0.3955) was positively associated (r = 0.4, 95% CI: 0.03 to 0.7) with Saccharomonospora sp.. In ovarian cancer patients, CA125 (correlation coefficient = -0.4451) was negatively correlated (r = -0.4, 95% CI: -0.7 to -0.09) with Porphyromonas sp.. In endometrial cancer patients, CEA (correlation coefficient = -0.3868) was negatively correlated (r = -0.4, 95% CI: -0.7 to -0.02) with Cutibacterium acnes. This study promoted our understanding of the intratumoral microbiome in gynecological malignancies.IMPORTANCEIn this study, we found the compositional spectrum of tumor microbes among gynecological malignancies were largely similar by sharing a few taxa and differentiated by substantial species owned uniquely. Certain species, mostly unreported, were identified to be associated with clinical characteristics. This study prompted our understanding of gynecological malignancies and offered evidence for tumor microbes affecting tumor biology among cancers in the female reproductive system.
期刊介绍:
Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.