添加精浆蛋白对犬精子体外动力学特性的影响

IF 0.6 4区 农林科学 Q3 VETERINARY SCIENCES Veterinarni Medicina Pub Date : 2022-04-28 eCollection Date: 2022-07-01 DOI:10.17221/73/2021-VETMED
Tsvetan Tsvetkov, Nadya Petrova, Denica Daskalova
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引用次数: 0

摘要

本研究的目的是评估添加不同分子量的精浆蛋白组分后,获能和去势犬精子的运动能力和动力学模式的变化。有人认为,精浆中的蛋白质有助于精子存活,并具有双重作用:获能和去势。对新鲜射精的精浆进行色谱分离,得到了四种蛋白质组分。通过计算机辅助精子分析,确定了精子亚群在与四种蛋白质馏分分别孵育后的特定运动和动力学特征。对对获能和去势有显著影响的组分进行了二维电泳。通过精子分类分析仪,在与高分子量的精浆蛋白组分培养的精子亚群中观察到了获能的变化,其头部侧向位移的曲线速度和振幅都很高,这也反映在线性度、直线度和渐进运动性的降低上。用低分子量精浆蛋白部分培养的精子亚群似乎经历了一个去势过程(曲线速度下降,线性度、直线度上升,并显示出渐进的运动性)。尽管精浆蛋白具有广泛的作用,但其在获能和去势方面的作用仍未确定。
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Addition of seminal plasma proteins effecting the in vitro kinetic properties of canine spermatozoa.

The objective of this study is to evaluate the changes in the motility and kinetic patterns of canine spermatozoa, capacitated and decapacitated, after the addition of seminal plasma protein fractions with different molecular weight. It has been proposed that proteins in seminal plasma support the survival of the spermatozoa and exert a dual effect: capacitation and decapacitation. The seminal plasma from fresh ejaculates was subjected to chromatographic separation and four protein fractions were obtained. Computer-assisted sperm analysis was used to determine the sperm subpopulations with specific motion and kinetic characteristics after incubation with each of the four protein fractions. Two-dimensional electrophoresis of the fractions that exhibit a significant effect on the capacitation and decapacitation was performed. By sperm class analyser, capacitation changes were observed in the sperm subpopulation with a high curvilinear velocity and amplitude of lateral head displacement incubated with the seminal plasma protein fraction with a high molecular weight, which was also reflected in the decreased linearity, straightness, and progressive motility. The sperm subpopulation incubated with the seminal plasma protein fraction with a low molecular weight seemed to undergo a process of decapacitation (decreasing of the curvilinear velocity, increasing of the linearity, straightness and showing progressive motility). Despite their ample panorama of actions, the role of seminal plasma proteins regarding capacitation and decapacitation is still undetermined.

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来源期刊
Veterinarni Medicina
Veterinarni Medicina Veterinary Sciences-兽医学
CiteScore
1.30
自引率
0.00%
发文量
62
审稿时长
18-36 weeks
期刊介绍: The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.
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