炎症性肠病患者的 MiR-146a (rs2910164) 基因多态性及其对循环 MiR-146a 水平的影响

IF 4.5 2区 医学 Q2 CELL BIOLOGY Inflammation Pub Date : 2024-08-06 DOI:10.1007/s10753-024-02108-0
Rasha Ahmed Ghorab, Shaimaa H Fouad, Ahmed F Sherief, Eman M El-Sehsah, Sara Shamloul, Sara I Taha
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引用次数: 0

摘要

微RNA-146a(miR-146a)与炎症性肠病(IBD)的病理生理学有关。然而,其确切过程仍不完全清楚。相互矛盾的研究表明,miR-146a 的表达可能受 miR-146a rs2910164 C > G 多态性的影响。本病例对照研究旨在探讨埃及 IBD 患者 miR-146a rs2910164 C > G 基因多态性的相关性及其对循环 miR-146a 表达水平的影响。我们纳入了 40 名 IBD 患者和 30 名匹配的健康对照组。对所有参与者进行了 miR-146a rs2910164 多态性基因分型,并使用定量实时 PCR 评估了 miR-146a 的表达水平。47% 的 IBD 患者和 70% 的 IBD 患者分别报告了 MiR-146a rs2910164 GG 基因型和 G 等位基因。它们与 IBD 风险的增加有关。所有 CC 基因型的 IBD 患者(100%)和大多数 CG 基因型的 IBD 患者(66.67%)的疾病均为非活动性,而大多数 GG 基因型的 IBD 患者(73.68%)的疾病为活动性。miR-146a 的表达水平在 CC 基因型中最高,在 GG 基因型中最低。此外,与对照组相比,IBD 患者的 miR-146a 表达水平明显下降,且随疾病活动而下降。粪便钙蛋白与 miR-146a 表达水平的联合检测提高了诊断灵敏度和阴性预测值,有助于区分疾病活动期和非活动期的 IBD 患者。我们的研究发现,在埃及人群中,miR-146a rs2910164 GG 基因型和 G 等位基因与 IBD 易感性和活动性增加密切相关。miR-146a rs2910164 多态性也会降低这些患者的 miR-146a 表达水平。对更大样本量和不同种族人群的进一步研究可能是建立这种遗传关联取得进展的关键。
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MiR-146a (rs2910164) Gene Polymorphism and Its Impact on Circulating MiR-146a Levels in Patients with Inflammatory Bowel Diseases.

MicroRNA-146a (miR-146a) has been involved in the pathophysiology of inflammatory bowel disease (IBD). However, the precise processes are still not entirely understood. Contradictory studies suggest that miR-146a expression could be influenced by the miR-146a rs2910164 C > G polymorphism. This case-control study aimed to investigate the association of miR-146a rs2910164 C > G gene polymorphism and its impact on circulating miR-146a expression levels in Egyptian IBD patients. We included 40 IBD patients and 30 matched healthy controls. Genotyping of miR-146a rs2910164 polymorphism and assessment of miR-146a expression level were done using quantitative real-time PCR in all participants. MiR-146a rs2910164 GG genotype and the G allele were reported in 47% and 70% of the IBD patient group, respectively. And they were associated with increased IBD risk. All the IBD patients with the CC genotype (100%) and most of those with the CG genotype (66.67%) had an inactive disease, while most IBD patients with the GG genotype (73.68%) had an active disease. The miR-146a expression level was the highest with the CC genotype and the lowest with the GG genotype. Also, miR-146a expression level decreased significantly in IBD patients than controls and with disease activity. Combined detection of fecal calprotectin with miR-146a expression level improved the diagnostic sensitivity and the negative predictive value in differentiating IBD patients with active disease from those inactive. Our study identified a strong association of miR-146a rs2910164 GG genotype and G allele with IBD-increased susceptibility and activity in the Egyptian population. The miR-146a rs2910164 polymorphism can reduce miR-146a expression levels in these patients as well. Further research on a larger sample size and different ethnic populations can be the key to progress in establishing this genetic association.

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来源期刊
Inflammation
Inflammation 医学-免疫学
CiteScore
9.70
自引率
0.00%
发文量
168
审稿时长
3.0 months
期刊介绍: Inflammation publishes the latest international advances in experimental and clinical research on the physiology, biochemistry, cell biology, and pharmacology of inflammation. Contributions include full-length scientific reports, short definitive articles, and papers from meetings and symposia proceedings. The journal''s coverage includes acute and chronic inflammation; mediators of inflammation; mechanisms of tissue injury and cytotoxicity; pharmacology of inflammation; and clinical studies of inflammation and its modification.
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