细胞周期蛋白 B3 是一种显性快速作用细胞周期蛋白,它能驱动早期胚胎的快速有丝分裂。

IF 7.4 1区 生物学 Q1 CELL BIOLOGY Journal of Cell Biology Pub Date : 2024-11-04 Epub Date: 2024-08-06 DOI:10.1083/jcb.202308034
Pablo Lara-Gonzalez, Smriti Variyar, Shabnam Moghareh, Anh Cao Ngoc Nguyen, Amrutha Kizhedathu, Jacqueline Budrewicz, Aleesa Schlientz, Neha Varshney, Andrew Bellaart, Karen Oegema, Lee Bardwell, Arshad Desai
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引用次数: 0

摘要

早期胚胎的有丝分裂通常以很快的速度进行,但这种速度是如何实现的尚不清楚。在这里,我们发现细胞周期蛋白 B3 是优雅小鼠胚胎快速有丝分裂的主要驱动力。细胞周期蛋白 B1 和 B2 支持缓慢的有丝分裂(从 NEBD 到无丝分裂 ∼600 秒),但细胞周期蛋白 B3 的存在主导性地推动了野生型中观察到的约三倍快的有丝分裂。在细胞周期蛋白 B1 和 B2 驱动的有丝分裂过程中,多个有丝分裂事件被减慢,细胞周期蛋白 B3 相关的 Cdk1 H1 激酶活性比细胞周期蛋白 B1 相关的 Cdk1 活性高 25 倍。在仅有细胞周期蛋白 B3 的快速有丝分裂中加入细胞周期蛋白 B1,会在染色体排列完成和无丝分裂开始之间产生 60 秒的延迟;这种延迟对分离的保真度非常重要,它依赖于无丝分裂激活剂 Cdc20 的抑制性磷酸化。因此,细胞周期蛋白 B3 的优势加上细胞周期蛋白 B1 依赖性延迟(通过 Cdc20 磷酸化起作用),为早期秀丽隐杆线虫胚胎的有丝分裂设定了快节奏,并确保了有丝分裂的保真度。
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Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses.

Mitosis in early embryos often proceeds at a rapid pace, but how this pace is achieved is not understood. Here, we show that cyclin B3 is the dominant driver of rapid embryonic mitoses in the C. elegans embryo. Cyclins B1 and B2 support slow mitosis (NEBD to anaphase ∼600 s), but the presence of cyclin B3 dominantly drives the approximately threefold faster mitosis observed in wildtype. Multiple mitotic events are slowed down in cyclin B1 and B2-driven mitosis, and cyclin B3-associated Cdk1 H1 kinase activity is ∼25-fold more active than cyclin B1-associated Cdk1. Addition of cyclin B1 to fast cyclin B3-only mitosis introduces an ∼60-s delay between completion of chromosome alignment and anaphase onset; this delay, which is important for segregation fidelity, is dependent on inhibitory phosphorylation of the anaphase activator Cdc20. Thus, cyclin B3 dominance, coupled to a cyclin B1-dependent delay that acts via Cdc20 phosphorylation, sets the rapid pace and ensures mitotic fidelity in the early C. elegans embryo.

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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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