没食子酸对戊唑醇诱导的成年白化大鼠小脑不良反应的改善作用:组织病理学和免疫组化证据。

IF 1.1 4区 医学 Q4 MICROSCOPY Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI:10.1080/01913123.2024.2387685
Omnia I Ismail, Hala Mohamed Hassanin
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引用次数: 0

摘要

戊唑醇(TEB)是一种常见的三唑类甾醇脱甲基化抑制剂杀菌剂,用于防治谷物、水果和蔬菜等作物的多种病害。这项研究的目的是评估 TEB 对成年白化大鼠小脑结构的影响,以及同时服用没食子酸(GA)可能产生的保护作用。随机选取四组四十只成年雄性白化大鼠,第一组每天灌胃玉米油,持续四周。第二组大鼠每天灌胃 100 毫克/千克剂量溶于生理盐水的 GA,连续 4 周;第三组大鼠每天灌胃 0.02 毫克/千克体重剂量溶于玉米油的 TEB,连续 4 周;第四组大鼠同时灌胃 TEB 和 GA。为了进行光学显微镜、超微结构和免疫组化研究,制备了小脑标本。暴露于 TEB 会导致神经元损伤,表现为具有空泡化胞质的浦肯野细胞变性、浦肯野细胞消失区域、篮状细胞出现空泡化并伴有神经髓变性、颗粒细胞聚集并在它们之间出现充血区域、小脑岛扩张、浦肯野细胞中出现弱阳性 bcl2 免疫反应以及大量 GFAP 阳性星形胶质细胞。GA减轻了TEB介导的小脑皮质组织学变化。我们的结论是,TEB 会导致大鼠小脑皮质中的浦肯野神经元退化和凋亡。GA对大鼠小脑皮质的TEB毒性具有神经保护作用。
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Ameliorative effects of gallic acid on tebuconazole-induced adverse effects in the cerebellum of adult albino rats: histopathological and immunohistochemical evidence.

Tebuconazole (TEB) is a common triazole sterol demethylation inhibitor fungicide utilized to manage a variety of diseases in crops like cereals, fruits, and vegetables. The aim of this work was to assess the effects of TEB on the structure of the cerebellum in adult albino rats and possible protective impact of co-administration of Gallic acid (GA). Four groups of forty adult male albino rats were randomly selected, and the rats in group I received corn oil through daily gavage for 4 weeks. Group II received GA dissolved in the normal saline at a dose of 100 mg/kg through daily gavage for 4 weeks, group III administered with TEB dissolved in corn oil at its acceptable daily intake dose (0.02 mg/kg body weight) through daily gavage for 4 weeks, group IV rats received both TEB and GA. For light microscopic, ultrastructural, and immunohistochemical investigations, cerebellar specimens were prepared. TEB exposure led to neuronal damage in the form of degenerated Purkinje cells with vacuolated cytoplasm, areas of lost Purkinje cells, the basket cells appeared vacuolated with degenerated neuropil, the granule cells clumped with congested areas between them, dilated cerebellar islands, weak positive bcl2 immunoreactions in the Purkinje cells, and numerous GFAP-positive astrocytes. GA mitigated TEB-mediated histological changes in the cerebellar cortex. We concluded that TEB caused Purkinje neurons in the rat cerebellar cortex to degenerate and undergo apoptosis. GA had a neuroprotective benefit against TEB toxicity in the rat cerebellar cortex.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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