{"title":"关于 Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) 的吸引力和产卵偏好的初步说明。","authors":"","doi":"10.1016/j.forsciint.2024.112170","DOIUrl":null,"url":null,"abstract":"<div><p><em>Chrysomya rufifacies</em> (Diptera: Calliphoridae) is a blow fly species of forensic importance, documented to have a strong preference for colonisation of substrate already inhabited by heterospecific blow fly larvae, thus exhibiting secondary colonisation behaviour. Larvae exhibit predatory behaviour that may be useful to support development where food substrate is limited or high competition exists, but they may alternately be drawn to pre-colonised substrate to capitalise on the advantages of collective exodigestion by previous/current colonisers. Previous authors have suggested female <em>Ch. rufifacies</em> may use visual orientation to detect substrate currently colonised by heterospecific larvae, rather than chemoreception of volatile organic compounds (VOCs), that signify condition of substrate, which would infer that active colonisation is likely a more important oviposition cue for <em>Ch. rufifacies</em> than substrate condition. This study addressed attraction as well as oviposition, examining whether the condition of substrate (either previously colonised or never colonised) or the presence of heterospecific larvae was more important in the initial choice of food source by female <em>Ch. rufifacies</em> where conspecifics were not present<em>,</em> and whether the condition of substrate and presence of heterospecific larvae affects the number of offspring deposited by a female. Attraction was studied using a Y-olfactometer system, and oviposition using a binary-choice assay, with females responding to pairwise choice between an array of meat conditions (fresh, larval aged or aged) and presence/absence of <em>Lucilia sericata</em> larvae. Females displayed a hierarchy of choice of larval aged substrate > aged substrate > fresh substrate, with the active presence of heterospecific larvae a secondary factor in choice. Females produced higher offspring numbers on meat that was either currently or previously colonised by heterospecific larvae, demonstrating the importance of heterospecific indicators of previous or current colonisation as an oviposition cue. This serves as an important consideration for entomologists working with <em>Ch. rufifacies</em> in any capacity where other blow fly species may be present, and most importantly for forensic entomologists where time of colonisation is utilised to estimate PMI.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0379073824002512/pdfft?md5=d15e6fed0d0701f2426c3c5c6dfdee56&pid=1-s2.0-S0379073824002512-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A preliminary note on attraction and oviposition preferences of Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae)\",\"authors\":\"\",\"doi\":\"10.1016/j.forsciint.2024.112170\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Chrysomya rufifacies</em> (Diptera: Calliphoridae) is a blow fly species of forensic importance, documented to have a strong preference for colonisation of substrate already inhabited by heterospecific blow fly larvae, thus exhibiting secondary colonisation behaviour. Larvae exhibit predatory behaviour that may be useful to support development where food substrate is limited or high competition exists, but they may alternately be drawn to pre-colonised substrate to capitalise on the advantages of collective exodigestion by previous/current colonisers. Previous authors have suggested female <em>Ch. rufifacies</em> may use visual orientation to detect substrate currently colonised by heterospecific larvae, rather than chemoreception of volatile organic compounds (VOCs), that signify condition of substrate, which would infer that active colonisation is likely a more important oviposition cue for <em>Ch. rufifacies</em> than substrate condition. This study addressed attraction as well as oviposition, examining whether the condition of substrate (either previously colonised or never colonised) or the presence of heterospecific larvae was more important in the initial choice of food source by female <em>Ch. rufifacies</em> where conspecifics were not present<em>,</em> and whether the condition of substrate and presence of heterospecific larvae affects the number of offspring deposited by a female. Attraction was studied using a Y-olfactometer system, and oviposition using a binary-choice assay, with females responding to pairwise choice between an array of meat conditions (fresh, larval aged or aged) and presence/absence of <em>Lucilia sericata</em> larvae. Females displayed a hierarchy of choice of larval aged substrate > aged substrate > fresh substrate, with the active presence of heterospecific larvae a secondary factor in choice. Females produced higher offspring numbers on meat that was either currently or previously colonised by heterospecific larvae, demonstrating the importance of heterospecific indicators of previous or current colonisation as an oviposition cue. This serves as an important consideration for entomologists working with <em>Ch. rufifacies</em> in any capacity where other blow fly species may be present, and most importantly for forensic entomologists where time of colonisation is utilised to estimate PMI.</p></div>\",\"PeriodicalId\":12341,\"journal\":{\"name\":\"Forensic science international\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0379073824002512/pdfft?md5=d15e6fed0d0701f2426c3c5c6dfdee56&pid=1-s2.0-S0379073824002512-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic science international\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0379073824002512\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic science international","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0379073824002512","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
A preliminary note on attraction and oviposition preferences of Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae)
Chrysomya rufifacies (Diptera: Calliphoridae) is a blow fly species of forensic importance, documented to have a strong preference for colonisation of substrate already inhabited by heterospecific blow fly larvae, thus exhibiting secondary colonisation behaviour. Larvae exhibit predatory behaviour that may be useful to support development where food substrate is limited or high competition exists, but they may alternately be drawn to pre-colonised substrate to capitalise on the advantages of collective exodigestion by previous/current colonisers. Previous authors have suggested female Ch. rufifacies may use visual orientation to detect substrate currently colonised by heterospecific larvae, rather than chemoreception of volatile organic compounds (VOCs), that signify condition of substrate, which would infer that active colonisation is likely a more important oviposition cue for Ch. rufifacies than substrate condition. This study addressed attraction as well as oviposition, examining whether the condition of substrate (either previously colonised or never colonised) or the presence of heterospecific larvae was more important in the initial choice of food source by female Ch. rufifacies where conspecifics were not present, and whether the condition of substrate and presence of heterospecific larvae affects the number of offspring deposited by a female. Attraction was studied using a Y-olfactometer system, and oviposition using a binary-choice assay, with females responding to pairwise choice between an array of meat conditions (fresh, larval aged or aged) and presence/absence of Lucilia sericata larvae. Females displayed a hierarchy of choice of larval aged substrate > aged substrate > fresh substrate, with the active presence of heterospecific larvae a secondary factor in choice. Females produced higher offspring numbers on meat that was either currently or previously colonised by heterospecific larvae, demonstrating the importance of heterospecific indicators of previous or current colonisation as an oviposition cue. This serves as an important consideration for entomologists working with Ch. rufifacies in any capacity where other blow fly species may be present, and most importantly for forensic entomologists where time of colonisation is utilised to estimate PMI.
期刊介绍:
Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law.
The journal publishes:
Case Reports
Commentaries
Letters to the Editor
Original Research Papers (Regular Papers)
Rapid Communications
Review Articles
Technical Notes.