光在拟南芥转录组对太空飞行环境的反应中起着主要作用。

IF 4.4 1区 物理与天体物理 Q1 MULTIDISCIPLINARY SCIENCES npj Microgravity Pub Date : 2024-08-06 DOI:10.1038/s41526-024-00417-0
Mingqi Zhou, Robert J Ferl, Anna-Lisa Paul
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引用次数: 0

摘要

拟南芥根吸引特性(CARA)太空飞行实验对同一太空飞行期间在光照和黑暗条件下生长的植物进行了转录组比较分析。CARA 比较了在国际空间站(ISS)上环境光和黑暗条件下生长的三种拟南芥基因型:Col-0、Ws 和 phyD(Col-0 背景下的植物色素 D 突变体)。在所有基因型中,叶片对太空飞行的反应比根尖有更多的差异表达基因(DEG),而且每个基因型都显示出太空飞行环境所特有的独特的光/暗转录组模式。Col-0叶片表现出明显的二分法,光照生长植株的太空飞行 DEGs 数量是黑暗生长植株的十倍。虽然 phyD 叶片中 DEGs 的总数与 Col-0 没有太大差别,但 phyD 改变了光照生长叶片对太空飞行的反应方式,许多与 Col-0 对太空飞行的生理适应有关的基因没有出现。这一结果与根尖形成了鲜明对比,之前的一项 CARA 研究表明,phyD 大大减少了根尖 DEGs 的数量。DEGs的数量很少,但有一系列空间改变的基因类别,这些基因类别在基因型和光照条件下都很常见。这种共性表明,关键的太空飞行基因与光照、防御和氧化应激反应的信号转导有关。然而,从 DEGs 中富集的这些关键信号通路在光照和黑暗条件下的太空飞行反应中显示出相反的调控方向,这表明在适应太空飞行过程中,光作为一种信号与光信号基因之间存在着复杂的相互作用。
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Light has a principal role in the Arabidopsis transcriptomic response to the spaceflight environment.

The Characterizing Arabidopsis Root Attractions (CARA) spaceflight experiment provides comparative transcriptome analyses of plants grown in both light and dark conditions within the same spaceflight. CARA compared three genotypes of Arabidopsis grown in ambient light and in the dark on board the International Space Station (ISS); Col-0, Ws, and phyD, a phytochrome D mutant in the Col-0 background. In all genotypes, leaves responded to spaceflight with a higher number of differentially expressed genes (DEGs) than root tips, and each genotype displayed distinct light / dark transcriptomic patterns that were unique to the spaceflight environment. The Col-0 leaves exhibited a substantial dichotomy, with ten-times as many spaceflight DEGs exhibited in light-grown plants versus dark-grown plants. Although the total number of DEGs in phyD leaves is not very different from Col-0, phyD altered the manner in which light-grown leaves respond to spaceflight, and many genes associated with the physiological adaptation of Col-0 to spaceflight were not represented. This result is in contrast to root tips, where a previous CARA study showed that phyD substantially reduced the number of DEGs. There were few DEGs, but a series of space-altered gene categories, common to genotypes and lighting conditions. This commonality indicates that key spaceflight genes are associated with signal transduction for light, defense, and oxidative stress responses. However, these key signaling pathways enriched from DEGs showed opposite regulatory direction in response to spaceflight under light and dark conditions, suggesting a complex interaction between light as a signal, and light-signaling genes in acclimation to spaceflight.

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来源期刊
npj Microgravity
npj Microgravity Physics and Astronomy-Physics and Astronomy (miscellaneous)
CiteScore
7.30
自引率
7.80%
发文量
50
审稿时长
9 weeks
期刊介绍: A new open access, online-only, multidisciplinary research journal, npj Microgravity is dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.
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